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We narrowed to 13 results for: pet-28

Showing: 1 - 13 of 13 results

  1. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...Younis, et al. Mol Cell Biol. 2010 Apr;30(7):1718-28. New and improved light-switchable bacterial TCSs...Unnatural amino acid incorporation in mammalian cells Peter Schultz and colleagues at the Scripps Research Institute...Giannaks et al 2003, Bert et al 2000). In 2000, Peter Cockerill and his lab developed an improved luciferase...available, as well as 5S RNA F30-2xdBroccoli fusion and pET28c-F30-Broccoli controls. Don’t just eat more broccoli...

  2. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...One Vector, Many Guises. Human Gene Therapy Methods 28:291–301 . https://doi.org/10.1089/hgtb.2017.084 Li...pseudoviruses for enveloped viruses. Rev Med Virol 28:e1963 . https://doi.org/10.1002/rmv.1963 Mazarakis... CR, Chu HY, Tortorici MA, Veesler D, Murphy M, Pettie D, King NP, Balazs AB, Bloom JD (2020) Protocol...

  3. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...total, the researchers applied the LITE system to 28 different genes to establish it as “a novel mode ...accompanying Nature News & Views Andreas Möglich and Peter Hegemann described several advantages of the new...

  4. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...Norway rat, from an obnoxious pest to a laboratory pet. ELife, 9. https://doi.org/10.7554/elife.50651 Vieira...10.1038/s41467-018-04899-x Yokoyama, Maho. (2020, May 28). E. coli as a Model Organism. News-Medical. Retrieved...

  5. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...CellTag libraries at three time points during the 28 day study: prior to reprogramming (CellTagMEF), at...CellTags for tagging two cell populations in a competitive transplant assay in the mouse intestine, demonstrating...

  6. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...Detection and Quantification. Volume 14. 2017, Pages 19-28, ISSN 2214-7535, https://doi.org/10.1016/j.bdq.2017.11.001...mutation detection, Nucleic Acids Research, Volume 28, Issue 19, 1 October 2000, Pages 3752–3761, https... into your data. Try different types of pipettes and pipetting methods to find what’s most consistent ...plate in a way that will tell you if you have pipetting errors on the edges of the plate. Compare the ...multiple configurations can help you identify pipetting or evaporation errors. And if time allows, you...BioRender.   Passive Reference Dyes Whatever your pipetting method, some variation will occur. A passive reference...

  7. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ... that interact with RNA/DNA hybrids. Genome Res., 28(9): 1405-1414 (2018). 10.1101/gr.237362.118 Chen,...particularly enriched in highly transcribed regions and repetitive regions, such as telomeres and centromeres. These...presumably due to their abundance of R-loops and repetitive sequence. All of the R-loops described above...

  8. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...CRISPR-Cas systems. 5. Nat Rev Microbiol. 2015 Sep 28. doi: 10.1038/nrmicro3569. PubMed PMID: 26411297 ... Up On Your Cpf1 Background Read About CRISPR Competitor NgAgo Learn How You Can Use piggyBac Vectors ...

  9. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...repeat sequences. Human gene therapy methods, 23 1, 18-28. PubMed PMID: 22428977. 2. Choi, V.W., Asokan, A....bacterial strains, like NEB Stable. NEB Stable competent cells lack RecA, a protein that aids in the homologous...

  10. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...immunosorbent for isolation of immunoglobulins. FEBS Letters 28:73–76 Additional resources on the Addgene blog: ...serum immunoglobulins indiscriminately, causing competition for binding sites. In cases like these, antibodies...

  11. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...Broader Impacts” requirements for certain grants) (28). As one young scientist, Matthew Niederhuber, said...References March for Science Website Locke, John, and Peter Laslett. Locke: Two treatises of government student... 15-008: Perspectives on Broader Impacts. The Pipettepen: The March for Science Raises Concerns Over Politicization...

  13. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...effector complex. Trends in Biochemical Sciences, 49(1), 28–37. https://doi.org/10.1016/j.tibs.2023.10.006 Tang...hypotheses that they are involved in plasmid competition or can hijack machinery from other CRISPR systems...CRISPR–Cas systems are highly diverse and involved in competition between plasmids. Nucleic Acids Research, 48(...
Showing: 1 - 13 of 13 results