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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 114 results for: Cre

Showing: 21 - 40 of 114 results

  1. Viral Production

    Type
    Collection
    ... the absence of Cre. mCherry expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from...were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at 1.7E6 viral genomes (vg)/cell...) alone at 1.1E6 vg/mL, or both. Two days later, Cre-dependent GFP expression was detected with direct...

  2. Zebrafish Plasmid Collection

    Type
    Collection
    ...resistant cell types. CreLite - George Eisenhoffer Lab. An optogenetically controlled Cre/loxP system that ...toolbox of genetic methods, such as Tol2 transposons, Cre-mediated site-specific recombination, morpholinos...fluorescent proteins, reporters, biosensors, and Cre expression plasmids. Large collections or kits are...that are amenable to genetics, high-throughput screening and non-invasive live imaging. A standard toolbox... Amro Hamdoun Lab. A useful “starter kit” for screening fluorescent protein fusion expression in organisms...antibodies to zebrafish cell surface receptors and secreted proteins. Return to top Other Resources Addgene...American Zebrafish Network (LAZEN) - LAZEN aims to increase the use of zebrafish as a model organism and to...

  3. Mammalian RNAi Tools

    Type
    Collection
    ...Addgene; browse by constitutive lentiviral shRNA, cre-regulated lentiviral shRNA, and more....expression as well as those that allow for conditional (Cre-lox) or inducible (Tet) expression are available....: Overview of shRNA-mediated RNA interference. Created with BioRender.com. shRNA Plasmids Browse our selection...

  4. Lentivirus Plasmids

    Type
    Collection
    ... pSico 3rd Conditional (Cre-lox), stable expression of shRNAs; addition of Cre turns on shRNA expression...expression. See article for more cre-lox shRNA expression variants. Jacks 19319 pLJM1-EGFP 3rd CMV-driven EGFP...systems available at Addgene have been designed for increased researcher safety. Read our Lentiviral Guide for...22502 pCEP4-tat N/A Packaging Expression of tat; increases viral titer Kasparov 22501 pHEF-VSVG N/A Envelope...

  5. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Expression Vectors page Cre-lox Site-specific recombination See our dedicated Cre-lox Plasmids page pBF3060...including plasmids for constitutive, conditional (Cre-lox), or inducible (Tet) expression miRNA and 3' ...bacterial expression (Gateway) pYD1 - Expression, secretion, and display of proteins on the extracellular ...some experiments, scientists often want to create stable cell lines in which the expression cassette... your regulatory element or gene of interest to create a luciferase reporter Promoter Measure promoter...

  6. AAV Viral Preps

    Type
    Collection
    ...Channelrhodopsins, Halorhodopsins, Archaerhodopsins Recombinases Cre, Flp, Dre Biosensors Calcium Sensors, Glutamate Sensors...

  7. Plasmid Collections

    Type
    Collection
    ...Genome Engineering Genome Engineering Overview CRISPR Cre-lox TALENs Zinc Fingers Luminescence Fluorescent ...

  8. Optogenetics Plasmid Collection

    Type
    Collection
    ...inhibition. For example, search for "ChR mCherry", "Cre inhibition", or other terms. Looking for viral preps...channelrhodopsin have been engineered or identified to increase the spectral and kinetic properties as well as...

  9. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...EGFP Del Bene pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2 74010 Zebrafish U6 yes, cut S. pyogenes Cre Del Bene pTE4396...pyogenes mCherry Kuhn AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR 60229 Mammalian/AAV SapI none S. pyogenes... pyogenes Zhang AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR 60231 Mammalian/AAV ...pyogenes Zhang AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR 60226 Mammalian/AAV SapI none S. pyogenes...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Plasmid Expression...

  10. Worm Expression Resources

    Type
    Collection
    ...RNAi, empty backbones, fluorescent proteins, and Cre expression plasmids. Large collections or kits are...

  11. Bacterial Expression Systems

    Type
    Collection
    ... Ashwani Kumar 134405 pBbAW4k-loxP-TT-loxP-mRFP1 Cre recombinase activity Fluorescence (mRFP1) Escherichia...our CRISPR Plasmids and Resources page. Check our Cre-Lox Plasmids Collection for a selection of plasmids...Guide CRISPR Guide Bacteria are commonly used to create, store, and replicate plasmids of all types, but...and an engineered strain for a B2H system used to screen the activity of arrays of zinc finger nucleases...benzoate (cumate) Sphingomonas sp. , Caulobacter crescentus , Paracoccus denitrificans , Methylobacterium...

  12. Neurodegeneration Research Collection

    Type
    Collection
    ...Noteworthy: AAV.rTH.PI.Cre.SV40 ready-to-use viral prep useful for dopamine research. CBA-driven, Cre-dependent...blood brain barrier integrity, and using CRISPR screens to understand more globally how neurons function...antibodies and scFvs for neuroscience research created with high-volume hybridoma sequencing on the NeuroMabSeq...community. This collection highlights plasmids created with support from the BRAIN Initiative. Jackson...Link opens in a new window) . This resource was created with support from the Chan Zuckerberg Initiative...

  13. CRISPR Plasmids - Tagging

    Type
    Collection
    ...vector assembly, and the remaining 5 plasmids include Cre and FLP expression vectors, a general cloning plasmid..._CEBPA_1 PX458_CEBPA_2 CREB1 Human FLAG pFETCh_CREB1 PX458_CREB_1 PX458_CREB_2 TGIF2 Human FLAG pFETCh_TGIF2...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...genome modification. This system can be used to create C- and N-terminal epitope tags. The plasmids in...has developed a systematic and scalable method to create marker-free mutations, insertions, and deletions...stem cell (hiPSC) lines. These cell lines were created using CRISPR and the donor plasmids containing ...

  14. CRISPR References and Information

    Type
    Collection
    ...pCFJ90 (pharyngeal co-injection marker); pDD104 (Cre recombinase) PDF, 105 KB Jaenisch CRISPR plasmid ...- a CRISPR Screen Tool (Link opens in a new window) Design gRNAs for custom CRISPR screens targeting a...in your lab, from knockouts to pooled library screening. CRISPR eBook Addgene's downloadable eBook about...CRISPR pooled libraries for large-scale functional screens. Available for knockout, activation, inhibition...For use with genome-scale CRISPR-Cas9 knockout screens (or GeCKO) technology. Developed by the Xiaole ...batch (Link opens in a new window) for lentiviral screens. sgRNA Scorer 2.0 (Link opens in a new window) ...considerations, and more. CasPEDIA is a community resource created and maintained by the Jennifer Doudna Lab . Read...

  15. Luciferase Plasmid Collection

    Type
    Collection
    ...PI 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla EF1α AAV-CRISPR knockout plasmid...promoter/enhancer regions. Blue/white screening can be used for screening of transformants in cloning process...kDa and 36 kDa, respectively). And they are not secreted, so cells must be lysed or the substrate must ...to measure luminescence. Gaussia luciferase is secreted and more stable, enabling time course experiments... kDa), very bright, and ATP-independent, and a secreted version of the protein is available, making it... regulatory element or gene of interest into to create a luciferase reporter. Unless otherwise noted, ...Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF) Firefly, Renilla Creating ORF-Firefly luciferase gene fusions. Renilla luciferase...

  16. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Lenti-sgAtm#1/Cre ATM CMV Ataxia telangiectasia Charles Swanton 173580 Lenti-sgAtm#2/Cre ATM CMV Ataxia...Lewy body dementia Tiago Outeiro 89643 Lenti-sgAtm/Cre ATM U6 Ataxia telangiectasia Monte Winslow 89755 ...T5 Parkinson's Suzanne Pfeffer 208392 Lenti-sgRNA-Cre-GpNLuc-sgmATM ATM Ataxia telangiectasia Gaorav Gupta...Spinocerebellar ataxia 19 James Trimmer 129409 Slc1a3-CreERT2 Targeting Vector SLC1A3 PGK Episodic ataxia Walker...

  17. CRISPR Guide

    Type
    Collection
    ...used for large scale edits. These proteins, like Cre recombinase or phage derived serine integrases, insert... GAT; increase nuclease fidelity SpCas9-NG - NG; increase in vitro activity SpG - NGN; increase nuclease...others work to increase Cas9’s proofreading capabilities. No matter the method, increased fidelity enzymes...backbone HypaCas9 - increase Cas9 proofreading and discrimination evoCas9 - decrease off-target effects...domains to expand PAM recognition, increase specificity, and decrease off-target effects Sniper-Cas9 - ...compatible with truncated gRNAs to increase specificity SuperFi-Cas9 - increases fidelity with reduced nuclease...integration of donor DNA. Genome-Wide Screens Using CRISPR In a genetic screen, a population of cells with mutations...

  18. Plan Your Experiment

    Type
    Collection
    ... mutant allele of a gene (point mutant)? Increase or decrease expression of a target gene? Once you have...High-fidelity Cas enzymes increase specificity Dual-nickase approach increases specificity but is less ...efficiency. In fact, cleavage efficiency may increase or decrease depending upon the specific nucleotides ... the general framework of a CRISPR experiment. Created with BioRender.com. Select Your Desired Genetic...mammalian cell lines Interfere Activate (CRISPRa) Increase gene expression without permanently modifying ...likely won’t be able to completely avoid them. To increase specificity, consider using an engineered Cas ...target in your samples to ensure there are no discrepancies with the reference sequence you used for gRNA...

  19. Immunology Research Plasmids and Resources

    Type
    Collection
    ...Upon activation, NK cells secrete perforin and granzyme B, two enzymes that create pores within an infected...LSIGN, MGC129964, MGC47866 CREB1 cAMP responsive element binding protein 1 CREB, MGC9284 CTSB cathepsin ...chromogranin A (parathyroid secretory protein 1) CGA CHGB chromogranin B (secretogranin 1) SCG1 CKLF chemokine-like... SDS, SWDS SCG2 secretogranin II (chromogranin C) CHGC, SN, SgII SCGB3A1 secretoglobin, family 3A, member...HIN1, LU105, MGC87867, PnSP-2, UGRP2 SCT secretin - SCTR secretin receptor SR SCYE1 small inducible cytokine... B cells mediate humoral immunity through the secretion of antibodies, and also function as antigen presenting...

  20. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ... Bauer's labs details the use of CRISPR/Cas9 to create genomic deletions in mammalian cell lines. Along... pair of chimeric single guide RNAs (sgRNAs) to create two double strand breaks (DSBs) at a locus in order...strategies for genes and non-coding elements. For creating a gene knockout, two sgRNA located within exons...be 24-mer oligos ( Table 4 ). Design Deletion Screening Primers Design one set of primers internal to ... for future plating. Plate the other half for screening and primer validation (see step 6 ). NOTE: This... allows for sufficient cell proliferation for screening genomic DNA (gDNA) for the intended deletion by...visible with ~2 mm diameter. Primer Validation and Screening for CRISPR/Cas9-Mediated Deletion Isolate gDNA...
Showing: 21 - 40 of 114 results