...Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant ORFs... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...linked to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and more....CBA-driven, Cre-dependent expression of diphtheria toxin receptor (DTR)–GFP fusion protein. Azim et al...bioRxiv. 2022 Dec 2. Use PiggyBac plasmids with tet-inducible expression of transcription factors for sensory...Subcellular Localization, Optogenetics Plasmids, and Chemogenetics Plasmids. New and Noteworthy: Explore NeuroPAL...more. Plasmids provided by the foundation can be found on our Michael J. Fox Foundation Plasmid Resource...

...was developed by Shannon Boye’s lab. Plasmid Information The plasmid below is available in each of the above...We provide high quality AAV preps from select plasmids in the repository. Browse the University of Florida... preparations were produced with the pTR-UF11 plasmid ( Addgene #157970 ). Viral preparations are formulated...above serotypes and buffers. Please click on the plasmid name for ordering information. ID Name Promoter...157970 pTR-UF11 chimeric CMV/Chicken Beta actin (CBA) GFP Control Sergei Zolotukhin Citation Information AAV2..., a Recombinant Adeno-Associated Virus Vector Expressing Retinoschisin. Hum Gene Ther Clin Dev . Sep;26...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....

...pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 CAG Archon1 EGFP Cre dependent 5 Boyden 115892 pAAV-Syn-Archon1-KGC-GFP-ER2 Syn Archon1...Archon1 EGFP Constitutive 8 Boyden 115893 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] Syn Archon1 EGFP Cre dependent...Packaging Service: Biosensor AAV Browse our AAV that express fluorescent biosensor proteins, including calcium...retrograde serotype, produced with the AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....HaloCaMP1a 138327 pAAV-synapsin-HaloCaMP1a-EGFP Syn HaloCaMP1a EGFP Constitutive 1, 9 Schreiter Calcium Sensor...HaloCaMP1b 138328 pAAV-synapsin-HaloCaMP1b-EGFP Syn HaloCaMP1b EGFP Constitutive 1, 9 Schreiter Dopamine Sensors...

...Purpose pCDH-EF1-DIO-copGFP 72253 Expresses copGFP under EF-1 promoter when Cre is expressed by another vector...72259 Expresses FLPe and tdTomato from the CB promoter pCDH-EF1-Fon-copGFP 72260 Expresses copGFP from ...pCDH-EF1-Nluc 73024 Express Nluc from the EF1 promoter pCDH-EF1-copGFP 73030 Expresses copGFP from the EF1 promoter...pCDH-EF1S-Nluc 73033 Expresses Nluc from a truncated EF1 promoter pCDH-EF1s-copGFP 73034 Expressed copGFP from a truncated...pAdx-CMV-copGFP 73346 Expresses copGFP from the CMV promoter pAdx-CMV-tdTomato 73347 Expresses tdTomato...pAdx-EF1-copGFP 73353 Expresses copGFP from the EF1 promoter pAdx-EF1-tdTomato 73354 Expresses tdTomato...pCDH-CB-copGFP-T2A-iCre 72256 Expresses copGFP and iCre under from the CB promoter pCDH-CB-iCre 72257 Express iCre under from...

...Addgene's Viral Plasmids and Resources page for more information. Plasmids Expressing Spike and Spike ...Accessory Plasmids Envelope and Packaging Plasmids Popular Lentiviral Envelope and Packaging Plasmids Popular...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for...vector expressing destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing firefly...circulating variants. The table below lists expression plasmids that are suitable for generating pseudotyped...reporter vector expressing firefly luciferase. pCMV-FLuc - Retroviral reporter vector expressing firefly luciferase...COVID-19 and Coronavirus Plasmids & Resources Ginkgo Bioworks COVID-19 Plasmid Collection Return to top...

...iChIP plasmid, expresses 3xFNLDD in mammalian cells 48972 3xFNLDD/pMXs-IG iChIP plasmid, expresses 3xFNLDD...scheme Fujii Lab Plasmids Individual plasmids can be ordered via the links below: ID Plasmid Description 47948...pMD20 iChIP plasmid, LexA-binding elements 48874 3xFNLDD/pCMV-7.1 iChIP plasmid, expresses 3xFNLDD in ...pMXs-puro iChIP plasmid, expresses 3xFNLDD in mammalian cells 51240 3xFLAG-dCas9/pMXs-puro Expresses 3xFLAG-dCas9... 47948 3xFLAG-dCas9/pCMV-7.1 enChIP plasmid, expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis...pMXs-puro Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat. A retroviral expression vector...-neo Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat. A retroviral expression vector...

...zebrafish Guide RNA expression plasmids for the EGFP Reporter Gene Guide RNA expression plasmids for endogenous...CRISPR-Cas/RGN expression plasmids for use in human cells CRISPR-Cas/RGN expression plasmids for use in zebrafish... Joung Lab CRISPR-Cas/RGN reagents: Cpf1 expression plasmids (AsCpf1 and LbCpf1) for use in mammalian ...endogenous human genes Guide RNA expression plasmids for endogenous zebrafish genes...RNA-Guided Nuclease (RGN) Plasmids Genome... Genome Engineering CRISPR Joung Lab CRISPR Plasmids Joung Lab CRISPR-Cas...CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools...

...direct virus infection specifically into cells that express the TVA viral receptor. Thus, by pseudotyping the...virus with EnvA, only neuronal cells engineered to express TVA will be infected. Schematic depicting steps...to the starter cell. (2) The starter cell now expresses TVA and G-protein. EnvA-pseudotyped RAVB is delivered... via G-protein receptors, which are naturally expressed at presynaptic nerve terminals. (4) Neurons projecting...G-protein coated RABV. These projection neurons do not express G-protein, and thus do not give rise to G-protein...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G Can be used to complement deletion-mutant rabies...

... Plasmid Collections Zebrafish Plasmids Zebrafish Plasmids and Resources... of the plasmids and tools available at Addgene for use in zebrafish. Browse Plasmids Plasmid Tools for...biosensors, and Cre expression plasmids. Large collections or kits are described below . ID Plasmid Description...for plasmids containing your zebrafish gene of interest? Search Addgene's collection for plasmids that...highlights plasmids that contain tools for use in zebrafish research. This includes plasmids for genome...CRISPR resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples...Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression plasmids - Keith Joung Lab CRISPR/Cas9-based conditional...

...nucleocapsid protein (Brian Geiss). Plasmids SARS-CoV-2 Plasmids Many plasmids containing SARS-CoV-2 sequences...distribute SARS-CoV-2 plasmids. The collection contains: Plasmids for E. coli and Yeast expression Spike protein...a new window) BST-LF expression plasmid from Andrew Ellington's lab. Find plasmid tools that may be helpful...Addgene will maintain this plasmid collection page, which highlights plasmids available that may be useful...SARS-CoV-2 nucleocapsid protein. SARS-CoV-2 Plasmids : Plasmids that are available or coming soon containing...containing SARS-CoV-2 sequences. SARS-CoV Plasmids : Plasmids that are available containing SARS-CoV (SARS ...-2 infection. General Tools : Plasmids expressing Cas12a/Cas13a, Reverse Transcriptases, etc. that may...

... like... Keith Joung Lab plasmids Daniel Voytas Lab plasmids CRISPR plasmids The Joung Lab has developed...and to simplify the construction of TALEN expression plasmids using REAL, REAL-Fast or FLASH assembly. ...TALEN Kit Individual REAL TALE Repeat Plasmids TALEN Expression Vectors for REAL, REAL-Fast and FLASH ...Endogenous Human Genes TALENs for the EGFP Reporter Gene Additional Plasmids compatible with the REAL Assembly...REAL or REAL-Fast assembly process or lists of plasmid ID numbers required for FLASH assembly. Links to...FLASH TALE Transcriptional Activator Expression Vectors for REAL, REAL-Fast and FLASH TALENs for Endogenous...Assembly TALEN Kit: TALE-LSD1 plasmids for epigenome editing...

...Collections Richard Kahn Lab Plasmids GTPase Plasmids GAP and GEF Plasmids The ARF (ADP-ribosylation factor...examples of plasmid types available for each gene. Click the gene name for a full list of plasmids available... have plasmids available for request. Family Gene Entrez Gene ID Length (aa) Example Expression Systems...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...ARF family plasmids. ARF family members include regulatory GTPases, GEFs, and GAPs. Plasmid...

...multiple gRNAs using a single plasmid ensures that all gRNAs will be expressed in the same cell. This is ...tools require constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects... DNA. This sequence is cloned into a gRNA expression plasmid but does NOT include the PAM sequence or ...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science...sequences . Once you’ve selected your target, you’ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein...the DSB site. Therefore, a population of cells expressing Cas9 and a gRNA will result in a diverse array... cell must be validated experimentally. Browse Plasmids: Double-Strand Break (Cut) Multiplex Genome Engineering...

... Plasmid Collections Pleiades Promoter Plasmids Pleiades Promoter Project ...using the links to view individual plasmid webpages. These plasmids are described in Portales-Casamar ...Plasmids from the Pleiades Promoter Project - A regulatory toolbox of MiniPromoters to drive selective...selective expression in the brain. Plasmid...human MiniPromoters. Browse the Pleiades Promoter Plasmids using the table below. Additional information ...regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A. 2010 ... pEMS1301 cre/EGFP/NLS N/A pEMS1308 EGFP/cre N/A pEMS1302 EGFP/cre/NLS N/A pEMS1306 EGFP/NLS N/A pEMS1307...