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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 113 results for: LEA

Showing: 41 - 60 of 113 results

  1. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...downstream of the sequence to be cleaved. This non-specific cleavage is thought to activate programmed...DNA. In bacteria, once they have recognized and cleaved the target RNA sequence, they adopt an enzymatically...enzymatically active state and can bind and cleave additional RNAs regardless of homology to the crRNA. This...

  2. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...cloning grade DNA, please let us know using the Cloning Grade DNA Suggestion Form . Please note that this ... (± 5 ng/µl) Buffer 10 mM Tris-Cl, pH 8.5 Price Please log in to your account and check the individual...I find cloning resources or scientific support? Please see our Addgene Protocols for resources you may...

  3. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...Hochschild and colleagues ( Dove et al., Nature 1997 ) and later modified by Pabo and colleagues ( Joung et...this Kit These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite...

  4. Antibody Production

    Type
    Collection
    ...Lab on the antibody webpage. To learn more about these antibodies, please visit the Institute for Protein...mammalian cells. To learn which specific QC experiments were performed on your lot, please email [email protected]...

  5. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...reports have shown that engineered zinc finger nucleases (ZFNs) are effective tools for making targeted...the spacer sequence between the half-sites (see Nuclease Expression Vectors for more details). IMPORTANT...open-source' engineering of customized zinc-finger nucleases for highly efficient gene modification. Maeder...mutagenesis in zebrafish using customized zinc-finger nucleases. Foley JE et al. Nat Protoc. . 2009. Dec 3. 4(...

  6. Viral Vectors

    Type
    Collection
    ...Which Type of Virus Should I Use? Use this guide to learn more about the different types of viruses that are...select plasmids are now available through Addgene, learn more here . Browse Viral Plasmids by Type Visit ...viruses are temporary in the host. Use this guide to learn more about the different types of viruses that are...depending on the insert type and envelope used. Please consult with your institution’s Biosafety Committee...

  7. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Deisseroth AV-1-PV3248 105557-AAV1 pENN.AAV.CB7.CI.mCerulean.WPRE.RBG Control James M. Wilson AV-1-PV3508 98928...Wickersham AV-9-PV3248 105557-AAV9 pENN.AAV.CB7.CI.mCerulean.WPRE.RBG Control James M. Wilson AV-1-PV1090 105537...Plasmids are available for all the items listed below. Please let us know (Link opens in a new window) what you...you would like to see available in viral format. Please note this does not guarantee viral service, but...pENN.AAV.CB7.CI.TurboRFP.WPRE.RBG James M. Wilson Please contact [email protected] with any questions. Return...

  8. TALEN Plasmids and Kits

    Type
    Collection
    ...pathogenic Xanthomonas spp. to the FokI nuclease, TALENs bind and cleave DNA in pairs. Binding specificity ...DNA-binding domain of a TALE, along with strong nuclear localisation tags and repress transcription initiation... BioSciences (N153AA, C63AA) and showed robust cleavage activity in several later studies. pCAG-T7-TALEN...

  9. Caltech Systemic Capsids

    Type
    Collection
    ...mice use LY6A to cross the blood-brain barrier, leading to strain-specific tropism (See S3 table (Huang...review performance data of each of these serotypes, please refer to Addgene’s Data Hub and the Caltech CLOVER...using the Caltech serotypes in future publications, please acknowledge Viviana Gradinaru and cite as follows...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  10. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...modify a single genomic locus. Before ordering, please review the steps for using pooled CRISPR libraries...Genome-wide Subpool Viral Prep Available Yes No Clear Filters Name ID Library Type Species PI Lentiviral...Weissman 3rd 6 Varies Enriched subpools (kinase, nuclear, ribosomal, cell cycle) 51043 — 51048 Knockout ...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...

  11. Cre-lox system

    Type
    Collection
    ...Learn about the Cre-Lox system and how it can be used for genetic rearrangements and recombination. Use...Zeng 140135 pAAV-EF1a-iCreV Split Cre fused to nuclear localized wild-type VVD EF-1 alpha AAV Zeng 140284...expression of Cre Mouse Sncg AAV Hu 154018 LiOn-CMV∞Cre leak-proof iOn (LiOn) Cre switch CMV Mammalian Livet ...containing FLEx technology. Read the genOway press release for more information. Empty backbones to regulate...24334 hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean Heat-inducible reporter with Cre dependent H2B-RFP...

  12. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...designed to be used with any CRISPR nuclease and function. Please see the publication or plasmid information...and maintained by the Doudna Lab . gRNA Plasmids Please note that the following table lists both published...

  13. Retrovirus Plasmids

    Type
    Collection
    ...Learn about retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids...enter the nucleus during mitotic breakdown of the nuclear envelope). Additionally, γ-retroviruses integrate...integrate randomly into the host's genome, which can lead to oncogenesis (termed insertional mutagenesis). ...

  14. Chemogenetics AAV Preps

    Type
    Collection
    ...Serotype AAV1 AAV2 AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Receptor and Activity Reporter/Tag...available items found to match all requested criteria. Please consider using fewer filtering options. *AAVrg ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  15. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ..., additional nuclear localization signals, and two mutations in Cas9 to enhance nuclease activity; can...or Cas9 domains PE7 - addition of RNA-binding exonuclease protection factor La to PEmax to enhance pegRNA...

  16. Plant Plasmids and Resources

    Type
    Collection
    ...biology - deposit plant plasmids with Addgene! CRISPR Please refer to Addgene's CRISPR Guide for a general introduction...CRISPR/Cas Toolkit for Plants includes CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for...Plant Base Editors Save Time with Transient Plant Leaf Transformations RNA Interference in Plant Biology...

  17. Lentiviral Prep Service

    Type
    Collection
    ...Genomic Sequences To perform genome edits, Cas9 nuclease is used to generate double-stranded breaks ( DSBs...Suggestions Don’t see what you are looking for? Please let us know what you would like to see available... Suggestion Form (Link opens in a new window) . Please note this does not guarantee viral service, but...

  18. Zebrafish Plasmid Collection

    Type
    Collection
    ...site-specific recombination, morpholinos, zinc finger nucleases, TALENS, and CRISPR/Cas9-based gene editing make...Highlights of Zebrafish Tools in our Collection CRISPR Please refer to Addgene's CRISPR Guide for a general introduction...Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression plasmids - Keith Joung Lab CRISPR...

  19. Brain Armamentarium

    Type
    Collection
    ...10aa-H2B-WPRE3-BGHpA AAV vector for nuclear SYFP2 labeling in neurons. Useful for nuclear isolation and scRNA-seq ...SYFP2 (yellow fluorescent protein) plus separate nuclear FLAG epitope in striatal cholinergic neurons Jonathan...

  20. Tetracycline Inducible Expression

    Type
    Collection
    ...Learn about tetracycline-regulated gene expression and find plasmids for your experiments using tet- ...dox) is present, TetR binds to tetracycline and releases tet O, enabling transcription (Figure 1). While...tet-responsive systems: Tet-Off and Tet-On. Read on to learn more about the components of tet systems and how...
Showing: 41 - 60 of 113 results