Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 109 results for: LEA

Showing: 41 - 60 of 109 results

  1. AAV Molecular Tools

    Type
    Collection
    ...-pre-mGRASP-mCerulean CAG-driven, constitutive Expression of pre-synaptic mGRASP-mCerulean for synaptic...Genome Engineering Tools These AAV encode Cas9 nuclease used in the CRISPR-Cas9 system for targeted genome...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  2. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...reports have shown that engineered zinc finger nucleases (ZFNs) are effective tools for making targeted...the spacer sequence between the half-sites (see Nuclease Expression Vectors for more details). IMPORTANT...open-source' engineering of customized zinc-finger nucleases for highly efficient gene modification. Maeder...mutagenesis in zebrafish using customized zinc-finger nucleases. Foley JE et al. Nat Protoc. . 2009. Dec 3. 4(...

  3. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...pCS2plus-mCerulean 156-239-GGGS-TDP-43 TARDBP mCerulean CMV ALS Marco Morsch 162618 pCS2plus-mCerulean 156-...Parkinson's, FTD Michael Davidson 55431 mCerulean3-MAPTau-C-10 MAPT mCerulean3 CMV Parkinson's, FTD Michael Davidson...Sidhu 110061 pDUET-1-alpha-synuclein-mCerulean3-His6 SNCA His, mCerulean3 T7 Parkinson's Dan Mulvihill 110486...Lippincott-Schwartz 164214 PLEX-PGK-ANXA11-mCerulean ANXA11 mCerulean PGK ALS Jennifer Lippincott-Schwartz ...mRuby CMV ALS Jennifer Lee 216227 TDP-43 mCerulean TARDBP mCerulean CMV ALS Jennifer Lee 216709 pCAG-TSF-...If you do not see a particular gene of interest, please search our full plasmid collection using the search...Bacterial Yeast Other Promoter CMV T7 polH GAL Other Clear Filters ID Plasmid Name Gene Tags Promoter Disease...

  4. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Deisseroth AV-1-PV3248 105557-AAV1 pENN.AAV.CB7.CI.mCerulean.WPRE.RBG Control James M. Wilson AV-1-PV3508 98928...Wickersham AV-9-PV3248 105557-AAV9 pENN.AAV.CB7.CI.mCerulean.WPRE.RBG Control James M. Wilson AV-1-PV1090 105537...Plasmids are available for all the items listed below. Please let us know (Link opens in a new window) what you...you would like to see available in viral format. Please note this does not guarantee viral service, but...pENN.AAV.CB7.CI.TurboRFP.WPRE.RBG James M. Wilson Please contact [email protected] with any questions. Return...

  5. TALEN Plasmids and Kits

    Type
    Collection
    ...pathogenic Xanthomonas spp. to the FokI nuclease, TALENs bind and cleave DNA in pairs. Binding specificity ...DNA-binding domain of a TALE, along with strong nuclear localisation tags and repress transcription initiation... BioSciences (N153AA, C63AA) and showed robust cleavage activity in several later studies. pCAG-T7-TALEN...

  6. Luciferase Plasmid Collection

    Type
    Collection
    ...Luciferase catalyzes the oxidation of a substrate, leading to the emission of photons. Its robust signal, ...sensitivity, wide dynamic range, and resistance to photobleaching have made luciferase a popular tool for scientists...making it ideal for a variety of applications. To learn more and plan your experiment, check the plasmid's...Artificial luciferases desined de novo using deep-learning approaches. Split Luciferase Complementation assays...

  7. Caltech Systemic Capsids

    Type
    Collection
    ...mice use LY6A to cross the blood-brain barrier, leading to strain-specific tropism (See S3 table (Huang...review performance data of each of these serotypes, please refer to Addgene’s Data Hub and the Caltech CLOVER...using the Caltech serotypes in future publications, please acknowledge Viviana Gradinaru and cite as follows...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  8. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...modify a single genomic locus. Before ordering, please review the steps for using pooled CRISPR libraries...Genome-wide Subpool Viral Prep Available Yes No Clear Filters Name ID Library Type Species PI Lentiviral...Weissman 3rd 6 Varies Enriched subpools (kinase, nuclear, ribosomal, cell cycle) 51043 — 51048 Knockout ...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...

  9. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...designed to be used with any CRISPR nuclease and function. Please see the publication or plasmid information...and maintained by the Doudna Lab . gRNA Plasmids Please note that the following table lists both published...

  10. Retrovirus Plasmids

    Type
    Collection
    ...Learn about retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids...enter the nucleus during mitotic breakdown of the nuclear envelope). Additionally, γ-retroviruses integrate...integrate randomly into the host's genome, which can lead to oncogenesis (termed insertional mutagenesis). ...

  11. Chemogenetics AAV Preps

    Type
    Collection
    ...Serotype AAV1 AAV2 AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Receptor and Activity Reporter/Tag...available items found to match all requested criteria. Please consider using fewer filtering options. *AAVrg ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  12. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ..., additional nuclear localization signals, and two mutations in Cas9 to enhance nuclease activity; can...or Cas9 domains PE7 - addition of RNA-binding exonuclease protection factor La to PEmax to enhance pegRNA...

  13. Plant Plasmids and Resources

    Type
    Collection
    ...biology - deposit plant plasmids with Addgene! CRISPR Please refer to Addgene's CRISPR Guide for a general introduction...CRISPR/Cas Toolkit for Plants includes CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for...Plant Base Editors Save Time with Transient Plant Leaf Transformations RNA Interference in Plant Biology...

  14. Lentiviral Prep Service

    Type
    Collection
    ...Genomic Sequences To perform genome edits, Cas9 nuclease is used to generate double-stranded breaks ( DSBs...Suggestions Don’t see what you are looking for? Please let us know what you would like to see available... Suggestion Form (Link opens in a new window) . Please note this does not guarantee viral service, but...

  15. Zebrafish Plasmid Collection

    Type
    Collection
    ...site-specific recombination, morpholinos, zinc finger nucleases, TALENS, and CRISPR/Cas9-based gene editing make...Highlights of Zebrafish Tools in our Collection CRISPR Please refer to Addgene's CRISPR Guide for a general introduction...Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression plasmids - Keith Joung Lab CRISPR...

  16. Brain Armamentarium

    Type
    Collection
    ...10aa-H2B-WPRE3-BGHpA AAV vector for nuclear SYFP2 labeling in neurons. Useful for nuclear isolation and scRNA-seq ...SYFP2 (yellow fluorescent protein) plus separate nuclear FLAG epitope in striatal cholinergic neurons Jonathan...

  17. Plasmids for Stem Cell Research

    Type
    Collection
    ...plasmids The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis. Nat Commun...and Sox2 Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental Potential of iPSCs. Cell Stem...Baldwin Fibroblasts iTSCs Lentiviral Mouse Extensive Nuclear Reprogramming Underlies Lineage Conversion into...

  18. Tetracycline Inducible Expression

    Type
    Collection
    ...Learn about tetracycline-regulated gene expression and find plasmids for your experiments using tet- ...dox) is present, TetR binds to tetracycline and releases tet O, enabling transcription (Figure 1). While...tet-responsive systems: Tet-Off and Tet-On. Read on to learn more about the components of tet systems and how...

  19. Biosensor AAV Preps

    Type
    Collection
    ...Serotype AAV1 AAV2 AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Promoter Sensor Label Activity Serotype...available items found to match all requested criteria. Please consider using fewer filtering options. *AAVrg ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  20. TREAT-AD Plasmid Collection

    Type
    Collection
    ...Consortium (SGC) Plasmids Haian Fu Lab plasmids Opher Gileadi Lab plasmids (Link opens in a new window) The TREAT-AD...AD and creating high-quality, well-characterized lead molecules for prioritized targets that are valued...
Showing: 41 - 60 of 109 results