...and aggressive plan to use gene editing, MECP2 reactivation, RNA editing, gene replacement therapy, RNA ...mutations in the MECP2 gene. Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR...diagnostic criteria have disease-causing mutations in the gene methyl-CpG binding protein 2 ( MECP2 ). MECP2 Rett...caused from loss-of-function mutations in the MECP2 gene. Causative mutations for Rett syndrome typically...global transcriptional regulator of thousands of genes and studies have suggested roles in transcriptional...activity dependent de-repression, chromatin remodeling, gene activation, and more. How loss of MECP2 protein ...suggests the major function of MECP2 is to regulate gene expression either locally or globally. The MECP2...

...going to bind on the chromosome. When dCas9 binds to a gene, it blocks the progression of the RNA polymerase...Tuning dCas9’s ability to block transcription enables robust, noiseless knockdown of bacterial genes. Molecular...of two genes without cross‐talk. (A) Schematic of the strain expressing two reporters and dCas9 under ...simultaneously. By fusing sfGFP and/or mCherry to genes of interest, it is thus possible to study the effect... protein dCas9 is expressed along a CRISPR array that contains 20 bp "addresses", where dCas9 is going...for quick integration of the aTc-inducible pTet-dCas9 in the attachment site of the phage 186. Detailed...

... ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter...Drosophila ID Plasmid Gene/Insert Promoter PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable...C. elegans ID Plasmid Gene/Insert Promoter PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or ...CRISPR plasmids for activating or upregulating gene expression in various model organisms. CRISPR...or regulatory regions of your gene of interest. If the plasmid that you choose does not also express a ...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...

... ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter...Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter...dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down gene expression...plasmids for transcriptional repression of target genes. CRISPR...with transcription. Design your gRNA to target your gene of interest’s promoter/enhancer or the beginning...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...

...transcription. Epigenetics dCas9 fused to an epigenetic modifier alters chromatin structure/gene expression...Regulation Activate dCas9 fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused...factors. gRNAs gRNA plasmids to target specific genes. Browse Plasmids by Model Organism Bacteria CRISPR...CRISPR? Experts cover CRISPR topics on Addgene's blog. Addgene's Viral Service : Request ready-to-use viral...Cas enzymes generate double strand breaks (DSBs). Nick Mutated Cas nickase enzymes generate single-strand...CRISPR plasmids available at Addgene. Search by species and CRISPR function. CRISPR...technologies are continously developing and expanding. Addgene works with leading scientists in the field to ensure...

...plasmids for epigenetic modification in mammalian or plant systems. Mammalian ID Plasmid Gene/Insert Promoter... target a specific promoter or enhancer for your gene of interest. Available modifications include: histone...Selectable Marker PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...targeted epigenetic modifications, researchers have fused catalytically dead dCas9 to epigenetic modifiers... CRISPR Epigenetics CRISPR Plasmids: Epigenetics Browse CRISPR Plasmids By Function...CRISPR plasmids for targeted epigenetic modification, including histone acetylation/demethylation, and...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...

...protein (FP) tags. Information on genes and associated neurodegenerative diseases was curated from several...sgHTT1-7sk-sgCas9 HTT U6, 7sk Huntington's Nicole Deglon 190901 pAAVss-U6-sgHTT51-7sk-Cas9 HTT U6, 7sk...but not exhaustive. If you do not see a particular gene of interest, please search our full plasmid collection... Huntington's Disease Parkinson's Disease Others Gene Type Wildtype Mutant Partial Sequence Codon-Optimized... T7 polH GAL Other Clear Filters ID Plasmid Name Gene Tags Promoter Disease PI 1177 p416 25Q GPD HTT GFP...Parkinson's Frank Soldner 180437 Px330-EGFP-LRRK2-CRISPR/Cas9 LRRK2 U6 Parkinson's Frank Soldner 180439 STR-KDEL_PGRN-SBP-mCherry... Neurodegeneration Neurodegeneration Plasmids Neurodegeneration Plasmid Collection Addgene has a ...

... ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter...Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert Promoter...elegans ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter... ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Xenopus ID Plasmid Gene/Insert Promoter...Selectable Marker PI Publication Parasites ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...

... ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter...Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert Promoter...Marker PI Publication Yeast ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication...CRISPR nickase plasmids for the generation of single-strand breaks. CRISPR...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

... ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Selectable...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...editing in mammalian systems. Mammalian ID Plasmid Gene/Insert PI Publication Do you have suggestions for...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...