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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 35 results for: MAK

Showing: 21 - 30 of 35 results

  1. Lentivirus Production

    Type
    Protocol
    ...endonuclease negative E. coli strain such as NEB stable. Make a mixture of a total of 500 μL PEI-OptiPro SFM with...-Tip There can be batch to batch variation when making the PEI working stock, therefore the ratio of μg...

  2. Pipetting Protocol

    Type
    Protocol
    ...plunger. Remove the entire pipette from the container making sure not to touch the sides of the container with...important for scientists to calibrate their pipettes to make sure that they are properly tuned to dispense the...

  4. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same time adding...restriction site (GAATTC) to the 5’ end of this primer, making our Forward Primer 5'-GAATTCATGTGGCATATCTCGAAGTAC...

  5. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...buffer. Fill the chamber with 1X MOPS running buffer. Make sure that the chamber is sealed. Load 5 µL of the...Pro-Tip Leaving clear lanes between samples will make quantifying the gel easier. Cover the electrophoresis...

  6. AAV Production in HEK293 Cells

    Type
    Protocol
    ...bottle up/down vigorously for 30 sec (it’s okay to make bubbles). Incubate at RT for 15 min. Note that longer...add the OptiMEM + DNA + PEI solution to the CS5. Make sure that all five layers are covered with media...

  7. CRISPR Library Amplification

    Type
    Protocol
    .... See below for options if the recombinant band makes up a significant proportion of the DNA pool. Last... weight of each pellet should be ~1-2 g. Pro-Tip Make sure to weigh the empty tube beforehand! If you’...

  9. Water Bath Protocol

    Type
    Protocol
    ...appropriate water level in the water bath so before use, make sure that there is enough water inside....
Showing: 21 - 30 of 35 results