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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 49 results for: ple

Showing: 41 - 49 of 49 results

  1. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...of DNA Samples Add an equal volume of TE-saturated phenol-chloroform to the aqueous DNA sample. Pro-Tip...colonies and to generate liquid bacterial cultures , please see those pages. Several companies, such as (Link...remaining contaminant proteins and RNase A from the DNA sample. When phenol is mixed with the aqueous solution...

  2. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ...before you place the sample at -80°C. Frozen tubes are hard to write on and samples stored for long periods...streaking onto LB agar will prevent it from thawing completely and will improve the shelf life. It is very important...

  3. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...bacteria containing the plasmids of interest (for example, fewer satellite colonies will grow). It is, however...over-agar for selection of transformed E. coli . Sample Data Selection of E.coli on LB-agar using different...of Carbenicillin stock used (150 µL per plate). Please note we have found that there is generally a broad...

  6. Weighing Reagents Protocol

    Type
    Protocol
    ...you can weigh out the reagents in batches. For example, if you need 300 g of sucrose, you can weigh out...

  7. Water Bath Protocol

    Type
    Protocol
    ...water baths with instructions on the bottle, for example, number of drops per liter. Place a thermometer...
Showing: 41 - 49 of 49 results