Vector Database
Welcome to Vector Database!
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
This vector is NOT available from Addgene.
Plasmid: pBP103
Source/Vendor: | ATCC |
Plasmid Type: | Unspecified |
Cloning Method: | Unknown |
Size: | 6200 |
Notes: | Generic chromosome fragmentation vector containing promoters for in vitro RNA synthesis. The unique cloning sites can be used to insert targeting sequences, such as cDNAs. [1] Any unique restriction site between the targeting sequence and the telomere-adjacent sequence can be used to linearize the plasmid before transformation. [1] The EcoRV and ClaI sites between the SP6 promoter and the telomere sequence can be used for recovery from deletion derivatives of YAC insert sequences adjacent to the fragmentation site. [1] A 0.24 kb EcoRI/SalI fragment containing the telomere-adjacent sequence from YTCA-1x was ligated into the MCS of pSP73. The EcoRI and BamHI sites of the MCS were destroyed and a 0.3 kb NdeI/PvuII fragment was inserted into the PvuII site of pRS303. [1] Restriction digests of the clone give the following sizes (kb): BamHI--4.6; EcoRI--4.6; SalI--4.6; SalI/EcoRI--4.4, 0.3. (ATCC staff) The order of the major features in this plasmid is: bla - HIS3 - lacZ'/MCS - HindIII - SphI - PstI - AccI - SalI - TEL - ClaI - EcoRV - SP6 promoter. [1] Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli, Saccharomyces cerevisiae YPH252, Saccharomyces cerevisiae CGY2516 [ATCC 74013]. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).) |
Catalog Number: | 77087 |
Stable: | Unspecified |
Constitutive: | Unspecified |
Viral/Non-Viral: | Unspecified |