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Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
This vector is NOT available from Addgene.
Generic chromosome fragmentation vector containing promoters for in vitro RNA synthesis. The unique cloning sites can be used to insert targeting sequences, such as cDNAs.  Any unique restriction site between the targeting sequence and the telomere-adjacent sequence can be used to linearize the plasmid before transformation.  The EcoRV and ClaI sites between the SP6 promoter and the telomere sequence can be used for recovery from deletion derivatives of YAC insert sequences adjacent to the fragmentation site.  A 0.24 kb EcoRI/SalI fragment containing the telomere-adjacent sequence from YTCA-1x was ligated into the MCS of pSP73. The EcoRI and BamHI sites of the MCS were destroyed and a 0.3 kb NdeI/PvuII fragment was inserted into the PvuII site of pRS303.  Restriction digests of the clone give the following sizes (kb): BamHI--4.6; EcoRI--4.6; SalI--4.6; SalI/EcoRI--4.4, 0.3. (ATCC staff) The order of the major features in this plasmid is: bla - HIS3 - lacZ'/MCS - HindIII - SphI - PstI - AccI - SalI - TEL - ClaI - EcoRV - SP6 promoter.  Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli, Saccharomyces cerevisiae YPH252, Saccharomyces cerevisiae CGY2516 [ATCC 74013]. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)