Vector Database

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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

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Plasmid: pCold-I

Information

Source/Vendor

Takara

Alt Name

pCOLD 1

Plasmid Type

Other, Cold shock expression vector

Promoter

cspA

Cloning Method

Unknown

Size

4407

5' Sequencing 1 Primer

pCold-Fwd

5' Sequencing 1 Primer Sequence

ACGCCATATCGCCGAAAGG

Tag 1

His6 (N-terminal)

Bacterial Resistance

Ampicillin

Catalog Number

3361

Stable

Unspecified

Constitutive

Unspecified

Viral/Non-Viral

Nonviral

Sequence

Copy sequence

AAGGAATGGT GTGGCCGATT AATCATAAAT ATGAAAAATA ATTGTTGCAT CACCCGCCAA 61 TGCGTGGCTT AATGCACATC AAATTGTGAG CGGATAACAA TTTGATGTGC TAGCGCATAT 121 CCAGTGTAGT AAGGCAAGTC CCTTCAAGAG TTATCGTTGA TACCCCTCGT AGTGCACATT 181 CCTTTAACGC TTCAAAATCT GTAAAGCACG CCATATCGCC GAAAGGCACA CTTAATTATT 241 AAGAGGTAAT ACACCATGAA TCACAAAGTG CATCATCATC ATCATCATAT CGAAGGTAGG 301 CATATGGAGC TCGGTACCCT CGAGGGATCC GAATTCAAGC TTGTCGACCT GCAGTCTAGA 361 TAGGTAATCT CTGCTTAAAA GCACAGAATC TAAGATCCCT GCCATTTGGC GGGGATTTTT 421 TTATTTGTTT TCAGGAAATA AATAATCGAT CGCGTAATAA AATCTATTAT TATTTTTGTG 481 AAGAATAAAT TTGGGTGCAA TGAGAATGCG CAGGCCCTTT CGTCTCGCGC GTTTCGGTGA 541 TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC 601 GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG 661 CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA AAATTGTAAA 721 CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA 781 ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGCCCGAGA TAGGGTTGAG 841 TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG 901 GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCA AATCAAGTTT 961 TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG 1021 AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC 1081 GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC 1141 GCTTAATGCG CCGCTACAGG GCGCGTACTA TGGTTGCTTT GACGTATGCG GTGTGAAATA 1201 CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGTC AGGTGGCACT TTTCGGGGAA 1261 ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA 1321 TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC 1381 AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC 1441 ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT 1501 ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT 1561 TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG 1621 CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT 1681 CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG 1741 CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA 1801 AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG 1861 AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA 1921 TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC 1981 AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC 2041 CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA 2101 TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA 2161 GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA 2221 AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC 2281 ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC 2341 CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT 2401 CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC 2461 CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT 2521 TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT 2581 TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG 2641 CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA 2701 AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA 2761 CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG 2821 GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG 2881 AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC 2941 TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA 3001 ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATA GTCATGCCCC 3061 GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGAGATCCCG 3121 GTGCCTAATG AGTGAGCTAA CTTACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT 3181 CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT 3241 TGCGTATTGG GCGCCAGGGT GGTTTTTCTT TTCACCAGTG AGACGGGCAA CAGCTGATTG 3301 CCCTTCACCG CCTGGCCCTG AGAGAGTTGC AGCAAGCGGT CCACGCTGGT TTGCCCCAGC 3361 AGGCGAAAAT CCTGTTTGAT GGTGGTTAAC GGCGGGATAT AACATGAGCT GTCTTCGGTA 3421 TCGTCGTATC CCACTACCGA GATATCCGCA CCAACGCGCA GCCCGGACTC GGTAATGGCG 3481 CGCATTGCGC CCAGCGCCAT CTGATCGTTG GCAACCAGCA TCGCAGTGGG AACGATGCCC 3541 TCATTCAGCA TTTGCATGGT TTGTTGAAAA CCGGACATGG CACTCCAGTC GCCTTCCCGT 3601 TCCGCTATCG GCTGAATTTG ATTGCGAGTG AGATATTTAT GCCAGCCAGC CAGACGCAGA 3661 CGCGCCGAGA CAGAACTTAA TGGGCCCGCT AACAGCGCGA TTTGCTGGTG ACCCAATGCG 3721 ACCAGATGCT CCACGCCCAG TCGCGTACCG TCTTCATGGG AGAAAATAAT ACTGTTGATG 3781 GGTGTCTGGT CAGAGACATC AAGAAATAAC GCCGGAACAT TAGTGCAGGC AGCTTCCACA 3841 GCAATGGCAT CCTGGTCATC CAGCGGATAG TTAATGATCA GCCCACTGAC GCGTTGCGCG 3901 AGAAGATTGT GCACCGCCGC TTTACAGGCT TCGACGCCGC TTCGTTCTAC CATCGACACC 3961 ACCACGCTGG CACCCAGTTG ATCGGCGCGA GATTTAATCG CCGCGACAAT TTGCGACGGC 4021 GCGTGCAGGG CCAGACTGGA GGTGGCAACG CCAATCAGCA ACGACTGTTT GCCCGCCAGT 4081 TGTTGTGCCA CGCGGTTGGG AATGTAATTC AGCTCCGCCA TCGCCGCTTC CACTTTTTCC 4141 CGCGTTTTCG CAGAAACGTG GCTGGCCTGG TTCACCACGC GGGAAACGGT CTGATAAGAG 4201 ACACCGGCAT ACTCTGCGAC ATCGTATAAC GTTACTGGTT TCACATTCAC CACCCTGAAT 4261 TGACTCTCTT CCGGGCGCTA TCATGCCATA CCGCGAAAGG TTTTGCGCCA TTCGATGGTG 4321 TCCGGGATCT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG 4381 GTTGAGGCCG TTGAGCACCG CCGCCGC

Published Plasmid Map