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Vector Database

Welcome to Vector Database!

Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.

This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.

This vector is NOT available from Addgene.

Plasmid: pFLAG-CMV-1

Source/Vendor: Sigma
Analyze: Sequence
Plasmid Type: Mammalian Expression
Promotor: CMV
Expression Level: High
Cloning Method: Restriction Enzyme
Size: 4732
5' Sequencing 1 Primer: CMV-F
5' Sequencing 1 Primer Sequence: CGCAAATGGGCGGTAGGCGTG
5' Sequencing 2 Primer: hGH-pA-R
5' Sequencing 2 Primer Sequence: CCAGCTTGGTTCCCAATAGA
Tag 1: FLAG (N terminal)
Bacterial Resistance: Ampicillin
For transient expression with extracellular secretion of N-terminal FLAG fusion proteins. The Met-preprotrypsin leader sequence precedes the FLAG tag Vector for expression and secretion of N-terminally FLAG-tagged proteins in mammalian cells.
Catalog Number: E7273
Stable: Unspecified
Constitutive: Unspecified
Viral/Non-Viral: Unspecified

Generated Plasmid Map