Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pGRE5-1
Information
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 4000
- Notes
- Plasmid to be cotransfected with plasmids encoding steroid receptors such as pRShGR (ATCC 67200) and pRShMR (ATCC 67201) to provide a reporter gene for hormone-dependent transcriptional activation. [3] Contains the LTR from mouse mammary tumor virus (nt -142 to nt -109), sequences encoding chloramphenicol acetyltransferase, and SV40 splicing sequences. [3] Restriction digests of the clone give the following sizes (kb): EcoRI--4.2, 1.8; PvuII--5.95, 0.3; BglI--4.8, 1.2; HindIII--3.55, 2.45; PstI--6.0. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli, eukaryote cells. Related vectors: pGRE5-2, pGRE5CAT, pGRE5-1EBV, pGRE5-2EBV, pGRE5CATEBV. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified