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Vector Database


Welcome to Vector Database!

Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.

This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.

This vector is NOT available from Addgene.

Plasmid: pHG165

Source/Vendor: ATCC
Analyze: Sequence
Plasmid Type: Unspecified
Cloning Method: Unknown
Size: 3375
Notes:
Created by Moore, July 1995, under contract with NCBI. Enzymes that cut only once (not including polylinker): AatII, AvaI, BalI, ScaI. The HaeII sites flanking lac in pUC8 were changed to HpaI sites. pBR322 was modified by removing PstI & HincII sites in ampR gene and changing the EcoRI to HpaI. A HpaI cassette with lac & MCS was cloned at the HpaI of the modified pBR322. pHG165 enables selection for inserts on MacConkey agar. The lac gene and multiple cloning sites are easily removed with a HpaI digest. Plasmid vector with the lac gene and polylinker of pUC8 but with copy number control of pBR322. Restriction digests of the clone give the following sizes (kb): HindIII--3.5; HpaI--3.0, about 0.5. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli JM101, E.coli, E.coli JM series. Related vectors: pBR322, pUC8. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number: 37618
Stable: Unspecified
Constitutive: Unspecified
Viral/Non-Viral: Unspecified

Generated Plasmid Map

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