Welcome to Vector Database!
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
This vector is NOT available from Addgene.
Created by Moore, July 1995, under contract with NCBI. Enzymes that cut only once (not including polylinker): AatII, AvaI, BalI, ScaI. The HaeII sites flanking lac in pUC8 were changed to HpaI sites. pBR322 was modified by removing PstI & HincII sites in ampR gene and changing the EcoRI to HpaI. A HpaI cassette with lac & MCS was cloned at the HpaI of the modified pBR322. pHG165 enables selection for inserts on MacConkey agar. The lac gene and multiple cloning sites are easily removed with a HpaI digest. Plasmid vector with the lac gene and polylinker of pUC8 but with copy number control of pBR322. Restriction digests of the clone give the following sizes (kb): HindIII--3.5; HpaI--3.0, about 0.5. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli JM101, E.coli, E.coli JM series. Related vectors: pBR322, pUC8. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)