Vector Database
Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pKC203
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 15000
- Notes
- The hygromycin B and G418 resistance structural genes and control elements can be isolated on the 7.5 kb BglII fragment or the 2.5 kb SalI/BglII fragment. The 1.5 kb BglII/SacI fragment contains hygR. The 1.65 EcoRI/SalI fragment contains G418R. [2] The 2.5 kb SalI/BglII fragment contains internal sites for AvaI, PvuI, EcoRI, XhoI, BstEII (one site each) and PstI, SacI (2 sites each). [2] The 7.5 kb BglII fragment contains a prokaryotic replicon and can be self-ligated to form plasmid pSC701. [2] This is a vector with easily isolated hygromycin B and G418 resistance structural genes and control elements for use in constructing other plasmids. [2] Restriction digests of the clone give the following sizes (kb): EcoRI--23, 16; BglII--17, 12, 6.0, 4.7, 3.9; SalI--18, 15, 11. This pattern differs from the information supplied by the depositor. (ATCC staff) Medium is -1 1065 plus hygromycin B (200 ug/ml). Hosts: E.coli JR225, vertebrate cells, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 31912
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified