Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pLKC482
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 9900
- Notes
- Restriction digests of the clone give the following sizes (kb): EcoRI--10.2, BamHI--10.2, PstI--6.2, 4.4, HindIII--10.2. (ATCC staff) One of a series of lacZY fusion vectors (ATCC 37594-37596) that allow fusions in one of three reading frames and retain flanking DNA for homologous recombination into the E.coli genome. (personal communication) A 1.3 kb HindIII/NruI kanR fragment (with HindIII site filled in) was inserted at the NruI site of a lacZY fusion plasmid. The 6.3 kb lacZY-kanR cassette is released with SmaI. Medium is 1227 LB plus ampicillin. Hosts: E.coli TX528, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37596
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified