Vector Database

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Plasmid: pLR60

Information

Source/Vendor

ATCC

Plasmid Type

Unspecified

Cloning Method

Unknown

Size

3694

Notes

ATCC length is 3350 bp. Deposited by: Guillermina Lozano Convenient cloning vector allowing generation of a fusion protein with the DNA-binding domain of GAL4 (aa 3-147). [1] Multiple cloning sites present at both ends of the GAL4 segment permit in-frame fusions with the DNA-binding domain in the middle or at the C-terminus of the fusion product. [1] The order of the major features of this plasmid is: pMB1 ori - lacI (non-functional) - lacZ'/ - T3 promoter - SacI/MCS/SpeI - GAL4 - ClaI/MCS/KpnI - T7 promoter - /lacZ' - f1 ori - ampR. [1] Transcription from the T3 promoter gives the sense strand. [1] pBR322 was modified by deletion of bp 75-2352, destruction (by filling in) of the HindIII site, deletion of a 100 bp AatII/ClaI fragment (nt 4285 and 22 of pBR322), and inserting an XbaI linker into the latter deletion junction. [2] Restriction digests of the clone give the following sizes (kb): SalI--3.35; SacI/KpnI--2.8, 0.5; XhoI--3.1, 0.25. (ATCC staff) Medium is 1227 LB plus ampicillin. GAL4, which is expressed at low levels, encodes a positive regulator of the galactose inducible genes GAL1, GAL2, GAL7, GAL10 and MEL1. GAL4 and the genes it regulates are not linked. Regulation takes place at the transcriptional level. [1] presents data suggesting GAL4 may encode two overlapping proteins. However, the second smaller protein has not yet been identified in vivo. NCBI gi: 171557 Hosts: E.coli, E.coli HB101. Related vectors: pG525. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)

Catalog Number

77335

Stable

Unspecified

Constitutive

Unspecified

Viral/Non-Viral

Unspecified

Sequence

Copy sequence

GATCCCTTAAGTTTAAACAACAACAGCAAGCAGGTGTGCAAGACACTAGAGACTCCTAACATGATGTATGCCAATAAAACACAAGAGATAAACAACATTGCATGGAGGCCCCAGAGGGGCGATTGGTTTGGGTGCGTGAGCGGCAAGAAGTTTCAAAACGTCCGCGTCCTTTGAGACAGCATTCGCCCAGTATTTTTTTTATTCTACAAACCTTCTATAATTTCAAAGTATTTACATAATTCTGTATCAGTTTAATCACCATAATATCGTTTTCTTTGTTTAGTGCAATTAATTTTTCCTATTGTTACTTCGGGCCTTTTTCTGTTTTATGAGCTATTTTTTCCGTCATCCTTCCCCAGATTTTCAGCTTCATCTCCAGATTGTGTCTACGTAATGCACGCCATCATTTTAAGAGAGGACAGAGAAGCAAGCCTCCTGAAAGATGAAGCTACTGTCTTCTATCGAACAAGCATGCGATATTTGCCGACTTAAAAAGCTCAAGTGCTCCAAAGAAAAACCGAAGTGCGCCAAGTGTCTGAAGAACAACTGGGAGTGTCGCTACTCTCCCAAAACCAAAAGGTCTCCGCTGACTAGGGCACATCTGACAGAAGTGGAATCAAGGCTAGAAAGACTGGAACAGCTATTTCTACTGATTTTTCCTCGAGAAGACCTTGACATGATTTTGAAAATGGATTCTTTACAGGATATAAAAGCATTGTTAACAGGATTATTTGTACAAGATAATGTGAATAAAGATGCCGTCACAGATAGATTGGCTTCAGTGGAGACTGATATGCCTCTAACATTGAGACAGCATAGAATAAGTGCGACATCATCATCGGAAGAGAGTAGTAACAAAGGTCAAAGACAGTTGACTGTATCGATTGACTCGGCAGCTCATCATGATAACTCCACAATTCCGTTGGATTTTATGCCCAGGGATGCTCTTCATGGATTTGATTGGTCTGAAGAGGATGACATGTCGGATGGCTTGCCCTTCCTGAAAACGGACCCCAACAATAATGGGTTCTTTGGCGACGGTTCTCTCTTATGTATTCTTCGATCTATTGGCTTTAAACCGGAAAATTACACGAACTCTAACGTTAACAGGCTCCCGACCATGATTACGGATAGATACACGTTGGCTTCTAGATCCACAACATCCCGTTTACTTCAAAGTTATCTCAATAATTTTCACCCCTACTGCCCTATCGTGCACTCACCGACGCTAATGATGTTGTATAATAACCAGATTGAAATCGCGTCGAAGGATCAATGGCAAATCCTTTTTAACTGCATATTAGCCATTGGAGCCTGGTGTATAGAGGGGGAATCTACTGATATAGATGTTTTTTACTATCAAAATGCTAAATCTCATTTGACGAGCAAGGTCTTCGAGTCAGGTTCCATAATTTTGGTGACAGCCCTACATCTTCTGTCGCGATATACACAGTGGAGGCAGAAAACAAATACTAGCTATAATTTTCACAGCTTTTCCATAAGAATGGCCATATCATTGGGCTTGAATAGGGACCTCCCCTCGTCCTTCAGTGATAGCAGCATTCTGGAACAAAGACGCCGAATTTGGTGGTCTGTCTACTCTTGGGAGATCCAATTGTCCCTGCTTTATGGTCGATCCATCCAGCTTTCTCAGAATACAATCTCCTTCCCTTCTTCTGTCGACGATGTGCAGCGTACCACAACAGGTCCCACCATATATCATGGCATCATTGAAACAGCAAGGCTCTTACAAGTTTTCACAAAAATCTATGAACTAGACAAAACAGTAACTGCAGAAAAAAGTCCTATATGTGCAAAAAAATGCTTGATGATTTGTAATGAGATTGAGGAGGTTTCGAGACAGGCACCAAAGTTTTTACAAATGGATATTTCCACCACCGCTCTAACCAATTTGTTGAAGGAACACCCTTGGCTATCCTTTACAAGATTCGAACTGAAGTGGAAACAGTTGTCTCTTATCATTTATGTATTAAGAGATTTTTTCACTAATTTTACCCAGAAAAAGTCACAACTAGAACAGGATCAAAATGATCATCAAAGTTATGAAGTTAAACGATGCTCCATCATGTTAAGCGATGCAGCACAAAGAACTGTTATGTCTGTAAGTAGCTATATGGACAATCATAATGTCACCCCATATTTTGCCTGGAATTGTTCTTATTACTTGTTCAATGCAGTCCTAGTACCCATAAAGACTCTACTCTCAAACTCAAAATCGAATGCTGAGAATAACGAGACCGCACAATTATTACAACAAATTAACACTGTTCTGATGCTATTAAAAAAACTGGCCACTTTTAAAATCCAGACTTGTGAAAAATACATTCAAGTACTGGAAGAGGTATGTGCGCCGTTTCTGTTATCACAGTGTGCAATCCCATTACCGCATATCAGTTATAACAATAGTAATGGTAGCGCCATTAAAAATATTGTCGGTTCTGCAACTATCGCCCAATACCCTACTCTTCCGGAGGAAAATGTCAACAATATCAGTGTTAAATATGTTTCTCCTGGCTCAGTAGGGCCTTCACCTGTGCCATTGAAATCAGGAGCAAGTTTCAGTGATCTAGTCAAGCTGTTATCTAACCGTCCACCCTCTCGTAACTCTCCAGTGACAATACCAAGAAGCACACCTTCGCATCGCTCAGTCACGCCTTTTCTAGGGCAACAGCAACAGCTGCAATCATTAGTGCCACTGACCCCGTCTGCTTTGTTTGGTGGCGCCAATTTTAATCAAAGTGGGAATATTGCTGATAGCTCATTGTCCTTCACTTTCACTAACAGTAGCAACGGTCCGAACCTCATAACAACTCAAACAAATTCTCAAGCGCTTTCACAACCAATTGCCTCCTCTAACGTTCATGATAACTTCATGAATAATGAAATCACGGCTAGTAAAATTGATGATGGTAATAATTCAAAACCACTGTCACCTGGTTGGACGGACCAAACTGCGTATAACGCGTTTGGAATCACTACAGGGATGTTTAATACCACTACAATGGATGATGTATATAACTATCTATTCGATGATGAAGATACCCCACCAAACCCAAAAAAAGAGTAAAATGAATCGTAGATACTGAAAAACCCCGCAAGTTCACTTCAACTGTGCATCGTGCACCATCTCAATTTCTTTCATTTATACATCGTTTTGCCTTCTTTTATGTAACTATACTCCTCTAAGTTTCAATCTTGGCCATGTAACCTCTGATCTATAGAATTTTTTAAATGACTAGAATTAATGCCCATCTTTTTTTTGGACCTAAATTCTTCATGAAAATATATTACGAGGGCTTATTCAGAAGCTTCGCTCATATAACGAAAAAAAAGGGTTTGGATCGAACGTAATTGAGATTGATTAGTTAATACTCAAAATAAAACAGCTCCTACCACCAGTGTAAAGTAGAACGTTAATAGAGCAATGTCTTCAGACAAATCTATTGAGAAAAATACAGATACGATCGCCTCTGAAGTTCACGAAGGTGATAATCATTCGAATAATTTGGGTTCAATGGAGGAAGAGATAAAATCAACGCCATCAGACCAATATGAAGAGATAGCTATAATTCCAACTGAGCCCCTCCATTCGGACAAAGAACTAAATGACAAGCAACAAAGTTTAGGCCATGAAGCACCCACAAATGTATCAAGAGAAGAACCTATTGGGATC