Vector Database

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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

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This vector is not available from Addgene.

Plasmid: PMT2

Information

Source/Vendor

unknown

Plasmid Type

Mammalian Expression

Promoter

SV40, Ad MLP

Cloning Method

Unknown

Size

5100

Bacterial Resistance

Ampicillin

Notes

Transcription unit is composed of SV40 ori/enh, Ad MLP, TPL, IVS, DHFR, SV40 PolyA; VAI gene; pUC18 plasmid backbone. More information: See map in Kaufman et al., 1989 MCB 9(3):946-958. Note: The full sequence of the plasmid provided on this page is only 4976 bp, so it may not be entirely correct.

Stable

Unspecified

Constitutive

Unspecified

Viral/Non-Viral

Nonviral

Sequence

Copy sequence

AAGCTTTTTGCAAAAGCCTAGGCCTCCAAAAAAGCCTCCTCACTACTTCT GGAATAGCTCAGAGGCCGAGGCGGCCTCGGCCTCTGCATAAATAAAAAAA ATTAGTCAGCCATGGGGCGGAGAATGGGCGGAACTGGGCGGAGTTAGGGG CGGGATGGGCGGAGTTAGGGGCGGGACTATGGTTGCTGACTAATTGAGAT GCATGCTTTGCATACTTCTGCCTGCTGGGGAGCCTGGGGACTTTCCACAC CTGGTTGCTGACTAATTGAGATGCATGCTTTGCATACTTCTGCCTGCTGG GGAGCCTGGGGACTTTCCACACCCTAACTGACACACATTCCACAGGATCC GGTCGCGCGAATTTCGAGCGGTGTTCCGCGGTCCTCCTCGTATAGAAACT CGGACCACTCTGAGACGAAGGCTCGCGTCCAGGCCAGCACGAAGGAGGCT AAGTGGGAGGGGTAGCGGTCGTTGTCCACTAGGGGGTCCACTCGCTCCAG GGTGTGAAGACACATGTCGCCCTCTTCGGCATCAAGGAAGGTGATTGGTT TATAGGTGTAGGCCACGTGACCGGGTGTTCCTGAAGGGGGGCTATAAAAG GGGGTGGGGGCGCGTTCGTCCTCACTCTCTTCCGCATCGCTGTCTGCGAG GGCCAGCTGTTGGGCTCGCGGTTGAGGACAAACTCTTCGCGGTCTTTCCA GTACTCTTGGATCGGAAACCCGTCGGCCTCCGAACGGTACTCCGCCACCG AGGGACCTGAGCGAGTCCGCATCGACCGGATCGGAAAACCTCTCGACTGT TGGGGTGAGTACTCCCTCTCAAAAGCGGGCATGACTTCTGCGCTAAGATT GTCAGTTTCCAAAAACGAGGAGGATTTGATATTCACCTGGCCCGCGGTGA TGCCTTTGAGGGTGGCCGCGTCCATCTGGTCAGAAAAGACAATCTTTTTG TTGTCAAGCTTGAGGTGTGGCAGGCTTGAGATCTGGCCATACACTTGAGT GACAATGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGGT CCAACTGCAGGCGAGCCTGAATTCGGGGGGGGGGGGGGGGGGGACAGCTC AGGGCTGCGATTTCGCGCCAAACTTGACGGCAATCCTAGCGTGAAGGCTG GTAGGATTTTATCCCCGCTGCCATCATGGTTCGACCATTGAACTGCATCG TCGCCGTGTCCCAAAATATGGGGATTGGCAAGAACGGAGACCTACCCTGG CCTCCGCTCAGGAACGAGTTCAAGTACTTCCAAAGAATGACCACAACCTC TTCAGTGGAAGGTAAACAGAATCTGGTGATTATGGGTAGGAAAACCTGGT TCTCCATTCCTGAGAAGAATCGACCTTTAAAGGACAGAATTAATATAGTT CTCAGTAGAGAACTCAAAGAACCACCACGAGGAGCTCATTTTCTTGCCAA AAGTTTGGATGATGCCTTAAGACTTATTGAACAACCGGAATTGGCAAGTA AAGTAGACATGGTTGGATAGTCGGAGGCAGTTCTGTTTACCAGGAAGCCA TGAATCAACCAGGCCACCTCAGACTCTTTGTGACAAGGATCATGCAGGAA TTTGAAAGTGACACGTTTTTCCCAGAAATTGATTTGGGGAAATATAAACT TCTCCCAGAATACCCAGGCGTCCTCTCTGAGGTCCAGGAGGAAAAAGGCA TCAAGTATAAGTTTGAAGTCTACGAGAAGAAAGACTAACAGGAAGATGCT TTCAAGTTCTCTGCTCCCCTCCTAAAGCTATGCATTTTTATAAGACCATG GGACTTTTGCTGGCTTTAGATCATAATCAGCCATACCACATTTGTAGAGG TTTTACTTGCTTTAAAAAACCTCCCACACCTCCCCCTGAACCTGAAACAT AAAATGAATGCAATTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGG TTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTT CACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCAT GTCTGGATCCCCGGCCAACGGTCTGGTGACCCGGCTGCGAGAGCTCGGTG TACCTGAGACGCGAGTAAGCCCTTGAGTCAAAGACGTAGTCGTTGCAAGT CCGCACCAGGTACTGATATCCCACCAAAAAGTGCGGCGGCGGCTGGCGGT AGAGGGGCCAGCGTAGGGTGGCCGGGGCTCCGGGGGCGAGGTCTTCCAAC ATAAGGCGATGATCATCGATGCTAGACCGTGCAAAAGGAGAGCCTGTAAG CGGGCACTCTTCCGTGGTCTGGTGGATAAATTCGCAAGGGTATCATGGCG GACGACCGGGGTTCGAACCCCGGATCCGGCCGTCCGCCGTGATCCATGCG GTTACCGCCCGCGTGTCGAACCCAGGTGTGCGACGTCAGACAACGGGGGA GCGCTCCTTTTGGCTTCCTTCCAGGCGCGGCGGCTGCTGCGCTAGCTTTT TTGGCGAGCTCGAATTAATTCTGCATTAATGAATCGGCCAACGCGCGGGG AGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTC GCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGG CGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATG TGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCT GGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCG TTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCT TACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAG CTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATC CGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGT GCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAAC AGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTT TTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGA TCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCAC GTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATC CTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTA AACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAG CGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAG ATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGAT ACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGC CAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCC ATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGT TAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCAC GCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGG CGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGG TCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGG TTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGC TTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTAT GCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGC CACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGG CGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACC CACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTT CTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGG GCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTG AAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTA TTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTG CCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAA TAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTG AAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAA GCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGG CGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGA GAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAA TACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGG GCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGAT GTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGA CGTTGTAAAACGACGGCCAGTGCC