Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pNASSbeta
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 6541
- Notes
- ATCC size is 6700 bp. The order of the major features in this plasmid is: pUC19 - EcoRI - SV40 late 16S/19S splice signals - NotI - lacZ - NotI - SV40 late polyadenylation signal - XbaI - HindIII - pUC19. [1] The lacZ gene may be excised by NotI and replaced with alternative sequences. [1] Promoter-less reporter plasmid permitting visual detection of transcription/translation activity by histochemical staining. [1] Promoters may be introduced upstream of the lacZ cassette at the unique EcoRI site. [1] Restriction digests of the clone give the following sizes (kb): EcoRI--6.7; HindIII--6.7; EcoRI/HindIII--4.0, 2.7; NotI--3.5, 3.2; BamHI--3.65, 2.85, 0.2. (ATCC staff) Medium is -1 TB plus ampicillin (200 ug/ml). Hosts: E.coli DH5alpha, E.coli, monkey COS cells, vertebrate cells. Related vectors: pBR322, pADbeta, pCMVbeta, pSVbeta, pTKbeta. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77180
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified