Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
Plasmid: pNF2
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 7800
- Notes
- The spcR marker is expressed in R. capsulatus. A wide range of transcription levels can be obtained by varying the nitrogen source in the medium. Transcription can be immediately stopped by the introduction of oxygen and ammonia. A Rhodobacter capsulatus expression vector with transcription of the insert under the control of the nifH promoter. Restriction digests of the clone give the following sizes (kb): SalI--8.2; BamHI--8.2; EcoRI/PstI--4.2, 3.6. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli, Rhodobacter. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37615
- GenBank
- M29400, M29401, M29402
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified