Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
Plasmid: pOJ31
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 5000
- Notes
- Medium copy number shuttle vector allowing lacZalpha complementation. Constructed from the cosmid vector pFD304 by addition of a lacZalpha-polylinker fragment and a 2.4 kb PstI-SalI fragment containing the JHJ-3 replicon. The JHJ-3 replicon permits replication in the following actinomycete genera: Streptomyces, Saccharopolyspora and Amycolatopsis. The order of the major features in the plasmid is: JHJ-3 ori - neo - ClaI* - synthetic promoter - pMB1 ori - cos site - lac promoter - SpeI/MCS/XbaI - lacZ. The ClaI* site is methylated, making the ClaI site in the polylinker unique in E. coli dam+ strains. [2] Growth medium: LB plus kanamycin (50 ug/ml)(ATCC number 1236) 37C Deposited by: C.V.Dery Hosts: E.coli, actinomycetes. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77416
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified