Vector Database

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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

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Plasmid: pPNT

Information

Plasmid Type

Mammalian Expression

Promoter

mPGK-1

Cloning Method

Unknown

Size

7331

Bacterial Resistance

Ampicillin

Selectable Marker

Neomycin

Notes

In the map, the shaded arrows represent the PGK-1 promoter and the hatched boxes represent the PGK-1 polyA addition sequences. This plasmid was published by Victor L.J. Tybulewicz, Camilla E. Crawford, Peter K. Jackson, Roderick T. Bronson, and Richard C. Mulligan in Cell Vol 65, p. 1153-1163 (June 28, 1991). Please refer to the article for more information.

Stable

Unspecified

Constitutive

Unspecified

Viral/Non-Viral

Unspecified

Sequence

Copy sequence

TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA 60 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG 120 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC 180 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC 240 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT 300 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT 360 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGCCAA GCTTCTGATG GAATTAGAAC 420 TTGGCAAAAC AATACTGAGA ATGAAGTGTA TGTGGAACAG AGGCTGCTGA TCTCGTTCTT 480 CAGGCTATGA AACTGACACA TTTGGAAACC ACAGTACTTA GAACCACAAA GTGGGAATCA 540 AGAGAAAAAC AATGATCCCA CGAGAGATCT ATAGATCTAT AGATCATGAG TGGGAGGAAT 600 GAGCTGGCCC TTAATTTGGT TTTGCTTGTT TAAATTATGA TATCCAACTA TGAAACATTA 660 TCATAAAGCA ATAGTAAAGA GCCTTCAGTA AAGAGCAGGC ATTTATCTAA TCCCACCCCA 720 CCCCCACCCC CGTAGCTCCA ATCCTTCCAT TCAAAATGTA GGTACTCTGT TCTCACCCTT 780 CTTAACAAAG TATGACAGGA AAAACTTCCA TTTTAGTGGA CATCTTTATT GTTTAATAGA 840 TCATCAATTT CTGCAGGTCC TGGCCGGGGT CCCGCGGAAA CTCGGCCGTG GTGACCAATA 900 CAAAACAAAA GCGCTCCTCG TACCAGCGAA GAAGGGGCAG AGATGCCGTA GTCAGGTTTA 960 GTTCGTCCGG CGGCGCCAGA AATCCGCGCG GTGGTTTTTG GGGGTCGGGG GTGTTTGGCA 1020 GCCACAGACG CCCGGTGTTC GTGTCGCGCC AGTACATGCG GTCCATGCCC AGGCCATCCA 1080 AAAACCATGG GTCTGTCTGC TCAGTCCAGT CGTGGACCTG ACCCCACGCA ACGCCCAAAA 1140 GAATAACCCC CACGAACCAT AAACCATTCC CCATGGGGGA CCCCGTCCCT AACCCACGGG 1200 GCCCGTGGCT ATGGCGGGCT TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC 1260 GAACTTGGGG GTTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT 1320 CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC 1380 GACCCAACAC CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 1440 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCAT CTCCCGGGCA AACGTGCGCG 1500 CCAGGTCGCA GATCGTCGGT ATGGAGCCTG GGGTGGTGAC GTGGGTCTGG ACCATCCCGG 1560 AGGTAAGTTG CAGCAGGGCG TCCCGGCAGC CGGCGGGCGA TTGGTCGTAA TCCAGGATAA 1620 AGACGTGCAT GGGACGGAGG CGTTTGGCCA AGACGTCCAA GGCCCAGGCA AACACGTTAT 1680 ACAGGTCGCC GTTGGGGGCC AGCAACTCGG GGGCCCGAAA CAGGGTAAAT AACGTGTCCC 1740 CGATATGGGG TCGTGGGCCC GCGTTGCTCT GGGGCTCGGC ACCCTGGGGC GGCACGGCCG 1800 TCCCCGAAAG CTGTCCCCAA TCCTCCCACC ACGACCCGCC GCCCTGCAGA TACCGCACCG 1860 TATTGGCAAG CAGCCCGTAA ACGCGGCGAA TCGCGGCCAG CATAGCCAGG TCAAGCCGCT 1920 CGCCGGGGCG CTGGCGTTTG GCCAGGCGGT CGATGTGTCT GTCCTCCGGA AGGGCCCCCA 1980 ACACGATGTT TGTGCCGGGC AAGGTCGGCG GGATGAGGGC CACGAACGCC AGCACGGCCT 2040 GGGGGGTCAT GCTGCCCATA AGGTATCGCG CGGCCGGGTA GCACAGGAGG GCGGCGATGG 2100 GATGGCGGTC GAAGATGAGG GTGAGGGCCG GGGGCGGGGC ATTTGAGCTC CCAGCCTCCC 2160 CCCCGATATG AGGAGCCAGA ACGGCGTCGG TCACGGCATA AGGCATGCCC ATTGTTATCT 2220 GGGCGCTTGT CATTACCACC GCCGCGTCCC CGGCCGATAT CTCACCCTGG TCGAGGCGGT 2280 GTTGTGTGGT GTAGATGTTC GCGATTGTCT CGGAAGCCCC CAGCACCTGC CAGTAAGTCA 2340 TCGGCTCGGG TACGTAGACG ATATCGTCGC GCGAACCCAG GGCCACCAGC AGTTGCGTGG 2400 TGGTGGTTTT CCCCATCCCG TGAGGACCGT CTATATAAAC CCGCAGTAGC GTGGGCATTT 2460 TCTGCTCCAG GCGGACTTCC GTGGCTTCTT GCTGCCGGCG AGGGCGCAAC GCCGTACGTC 2520 GGTTGCTATG GCCGCGAGAA CGCGCAGCCT GGTCGAACGC AGACGCGTGT TGATGGCAGG 2580 GGTACGAAGC CATACGCGCT TCTACAAGGC GCTTGCCAAA GAGGTGCGGG AGTTTCACGC 2640 CACCAAGATC TGCGGCACGC TGTTGACGCT GTTAAGCGGG TCGCTGCAGG TCGAAAGGCC 2700 CGGAGATGAG GAAGAGGAGA ACAGCGCGGC AGACGTGCGC TTTTGAAGCG TGCAGAATGC 2760 CGGGCCTCCG GAGGACCTTC GGGCGCCCGC CCCGCCCCTG AGCCCGCCCC TGAGCCCGCC 2820 CCCGGACCCA CCCCTTCCCA GCCTCTGAGC CCAGAAAGCG AAGGAGCAAA GCTGCTATTG 2880 GCCGCTGCCC CAAAGGCCTA CCCGCTTCCA TTGCTCAGCG GTGCTGTCCA TCTGCACGAG 2940 ACTAGTGAGA CGTGCTACTT CCATTTGTCA CGTCCTGCAC GACGCGAGCT GCGGGGCGGG 3000 GGGGAACTTC CTGACTAGGG GAGGAGTAGA AGGTGGCGCG AAGGGGCCAC CAAAGAACGG 3060 AGCCGGTTGG CGCCTACCGG TGGATGTGGA ATGTGTGCGA GGCCAGAGGC CACTTGTGTA 3120 GCGCCAAGTG CCCAGCGGGG CTGCTAAAGC GCATGCTCCA GACTGCCTTG GGAAAAGCGC 3180 CTCCCCTACC CGGTAGAATT CGAGCTCGGT ACCCGGGGAT CCTCTAGAGT CAGCTTCTGA 3240 TGGAATTAGA ACTTGGCAAA ACAATACTGA GAATGAAGTG TATGTGGAAC AGAGGCTGCT 3300 GATCTCGTTC TTCAGGCTAT GAAACTGACA CATTTGGAAA CCACAGTACT TAGAACCACA 3360 AAGTGGGAAT CAAGAGAAAA ACAATGATCC CACGAGAGAT CTATAGATCT ATAGATCATG 3420 AGTGGGAGGA ATGAGCTGGC CCTTAATTTG GTTTTGCTTG TTTAAATTAT GATATCCAAC 3480 TATGAAACAT TATCATAAAG CAATAGTAAA GAGCCTTCAG TAAAGAGCAG GCATTTATCT 3540 AATCCCACCC CACCCCCACC CCCGTAGCTC CAATCCTTCC ATTCAAAATG TAGGTACTCT 3600 GTTCTCACCC TTCTTAACAA AGTATGACAG GAAAAACTTC CATTTTAGTG GACATCTTTA 3660 TTGTTTAATA GATCATCAAT TTCTGCAGAC TTACAGCGGA TCGATCCCCT CAGAAGAACT 3720 CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA 3780 CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG 3840 CTATGTCCTG ATAGCGGTCC GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC 3900 GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT 3960 CGCCGTCGGG CATGCGCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT 4020 GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT 4080 CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC 4140 GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA 4200 GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT 4260 CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT 4320 CCTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT 4380 GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT 4440 AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCGGCCGATC 4500 CCATATTGGC TGCAGGTCGA AAGGCCCGGA GATGAGGAAG AGGAGAACAG CGCGGCAGAC 4560 GTGCGCTTTT GAAGCGTGCA GAATGCCGGG CCTCCGGAGG ACCTTCGGGC GCCCGCCCCG 4620 CCCCTGAGCC CGCCCCTGAG CCCGCCCCCG GACCCACCCC TTCCCAGCCT CTGAGCCCAG 4680 AAAGCGAAGG AGCAAAGCTG CTATTGGCCG CTGCCCCAAA GGCCTACCCG CTTCCATTGC 4740 TCAGCGGTGC TGTCCATCTG CACGAGACTA GTGAGACGTG CTACTTCCAT TTGTCACGTC 4800 CTGCACGACG CGAGCTGCGG GGCGGGGGGG AACTTCCTGA CTAGGGGAGG AGTAGAAGGT 4860 GGCGCGAAGG GGCCACCAAA GAACGGAGCC GGTTGGCGCC TACCGGTGGA TGTGGAATGT 4920 GTGCGAGGCC AGAGGCCACT TGTGTAGCGC CAAGTGCCCA GCGGGGCTGC TAAAGCGCAT 4980 GCTCCAGACT GCCTTGGGAA AAGCGCCTCC CCTACCCGGT AGAATTGACC TGCAGGGGCC 5040 CTCGAGCGGC CGCCACCGCG GTGGAGCTCC AGCTTTTGTT CCCTTTAGTG AGGGTTAATT 5100 GCGCGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA 5160 ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG 5220 AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG 5280 TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC 5340 TTTTCCGCTT CCTCGCTCAC TGACTCGCTA CGCTCGGTCG TTCGACTGCG GCGAGCGGTA 5400 CTGACTCACA CAAAAACGGT AACACAGTTA TCCACAGAAT CAGGGGATAA GGCCGGAAAG 5460 AACATGTGAG CAAAAGACCA GGAACAGGAA GAAGGCCACG TAGCAGGCGT TTTTCCATAG 5520 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TAGACGCTCA AGTCAGAGGT GGCGAAACCC 5580 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 5640 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 5700 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 5760 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 5820 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 5880 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 5940 CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 6000 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 6060 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 6120 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 6180 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 6240 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT 6300 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC 6360 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG 6420 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG 6480 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT 6540 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT 6600 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT 6660 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT 6720 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT 6780 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT 6840 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC 6900 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA 6960 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA 7020 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA 7080 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT 7140 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA 7200 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC 7260 TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG 7320 GCCCTTTCGT C

Published Plasmid Map