Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pREP4
Information
- Source/Vendor
- Invitrogen
- Plasmid Type
- Mammalian Expression
- Promoter
- RSV
- Expression Level
- Unknown
- Cloning Method
- Restriction Enzyme
- Size
- 10338
- 5' Sequencing 1 Primer
- pREP-F
- 3' Sequencing 1 Primer
- EBV-Rev
- Bacterial Resistance
- Ampicillin
- Selectable Marker
- Hygromycin
- Notes
- Hosts: E.coli NM522, E.coli INValphaF', mammal. Related vectors: EBV. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).) Episomal mammalian expression vector with Rous Sarcoma Virus long terminal repeat (RSV LTR) enhancer/promoter for transcription of recombinant genes cloned into the multiple cloning site. Plasmid contains the Epstein-Barr Virus replication origin (oriP) and nuclear antigen (encoded by the EBNA-1 gene) to permit extrachromosomal replication in human, primate and canine cells. Hygromycin B resistance gene can be used for stable selection in transfected cells.
- Stable
- Stable
- Constitutive
- Constitutive
- Viral/Non-Viral
- Unspecified