Vector Database
Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
Plasmid: pTAG-1
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 6000
- Notes
- Deposited by: Judith L. Fridovich-Keil The order of the major features in this plasmid is: SalI - SV40 polyadenylation trimer - XhoI/MCS/HindIII - beta-globin - PstI - SphI - ApaI - ampR/pGEM-7Zf(+). [1] Contains a trimer cassette of the SV40 major late polyadenylation signal to block background readthrough expression of the reporter gene. [1] A promoter/enhancer-cloning shuttle vector using human beta-globin as the reporter gene. Primarily useful for analysis of gene expression at the RNA level. [1] The ApaI site can be used for cloning enhancer elements. [1] Restriction digests of the clone give the following sizes (kb): PstI--3.8, 2.2; SphI--3.8, 2.2; HindIII--6.0; XhoI--6.0; BamHI--5.5, 0.55. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli, E.coli HB101, vertebrate cells. Related vectors: pbetaGlo4pA from pbetaGlo, from pbetaGloXH from pGEM7, & pJFCAT1, pBLCAT3. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77318
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified