Vector Database
Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pUR18
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 5500
- Notes
- The order of the major features in this plasmid is:ars1 - ura4 - HindIII/MCS/EcoRI/lacZ' - pMB1 ori - ampR. Constructed by inserting a 1.7 kb ClaI fragment containing ura4 and a 1.2 kb ClaI fragment containing ars1 into the NdeI site modified with a ClaI linker. Shuttle vector permitting visual detection of recombinants by beta-galactosidase alpha complementation. Restriction digests of the clone give the following sizes (kb): EcoRI--5.5; BamHI--5.5; PstI--5.5; HindIII--5.5. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli DH5alpha, Schizosaccharomyces pombe, E.coli. (Information source: <a href=http://seq.yeastgenome.org/vectordb target=_blank>VectorDB</a>.) Also, see Barbet, N., Muriel, W. J. and Carr, A. M. (1992). Versatile shuttle vectors and genomic libraries for use with Schizosaccharomyces pombe. Gene 114:59-66.
- Catalog Number
- 77297
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified
Published Plasmid Map
