Vector Database
Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pWL102
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 10500
- Notes
- Shuttle vector with demonstrated ability to transform Haloferax volcanii, Haloferax mediterranei (ATCC 33500), Halobacterium halobium, Haloarcula hispanica (ATCC 33960), and Haloarcula vallismortis (ATCC 29715). [1] The order of the major features in this plasmid is: NcoI - BamHI - HindIII - XbaI - KpnI - mevR - sphI - pMB1 ori - ampR - EcoRI - ClaI - HindIII - pHV2 fragment. [3] The 3.5 kb SphI/KpnI fragment conferring mevinolin resistance has been sequenced. The mutation in the hmgA gene is at position -29 relative to the transcription start site, and results in an increased level of transcription. [2] Restriction digests of the clone give the following sizes (kb): EcoRI--11.0; HindIII--6.6, 4.1; KpnI/SphI--7.2, 3.6; PstI--7.6, 3.0; XbaI--11.0. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli DH5alpha F', halobacteria, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77216
- GenBank
- M83531
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified