Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pXPRS-/pcDpolyB-
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 3753
- Notes
- + makes non-coding strand of gene. - makes coding strand of gene. Allows expression of cloned cDNAs in eukaryotic cells from the SV40 early region promoter. Contains the integenic region from bacteriophage f1 permitting synthesis of single-strand copies of the insert. pcDpolyB+ and pcDpolyB- differ in their orientations of the intergenic region from bacteriophage f1. Libraries constructed with each vector for a different cell type can be screened against each other for cell-specific sequences. A vector which maintains the cDNA cloning features of the Okayama-Berg vector while increasing the efficiency of making transformation-competent, cDNA-containing plasmids and allows enrichment by hybridization/selection. Restriction digests of the clone give the following sizes (kb): PstI--3.6; PvuII--uncut; KpnI--3.6; XbaI--3.6; KpnI/HindIII--3.15, 0.55. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, vertebrate cells, E.coli. Related vectors: pBR322, f1, pXPRS+. (Information source: <a href=http://seq.yeastgenome.org/vectordb target=_blank>VectorDB</a>.)
- Catalog Number
- 67727
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified