Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pYe CEN3 30
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 6300
- Notes
- Contains unique BamHI, BglII, XbaI, NruI, and SalI sites, with XbaI and BglII inactivating TRP1. (personal communication) Constructed by inserting a 627 bp BamHI/Sau3AI fragment containing CEN3 into the BamHI site of YRp7'. [2] Demonstrates mitotic stability increased over YRp7'; 60-90% of transformed S. cerevisiae J17 maintain the Trp+ phenotype after 20 generations. [2] Restriction digests of the clone give the following sizes (kb): BamHI--6.3; BglII--6.3; SalI--6.3; EcoRI--4.6, 1.7. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli JA228, Saccharomyces cerevisiae, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 53232
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified