Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: YCp50
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 7950
- Notes
- YCp50 was constructed by removing the EcoRI site in YCp19 between ARS1 and CEN4 and cloning the PvuII/HindIII fragment into the PvuII site of YIp5. The PvuII site was not regenerated. [2] YC-type shuttle vector. The order of the major features in this plasmid is: HindIII - BamHI - SphI - URA3 - CEN4 - ARS1 - pMB1 ori - bla. Medium is 1227 LB plus ampicillin. To pBR322, insert 1.1 kb HindIII-HindIII URA3 fragment into AvaI site with no overhangs and 10 G's added to nt 1428-1437 and 10 C's added to nt 2601-2610 [dG/dC tailing] to make YIp5. Ligate CEN4 from PvuII to EcoRI to ARS1 from EcoR1 to Hind III. Delete 190 nt around EcoRI site [85 nt from each side]. BalI site is deleted; perhaps it is intact in [2]. Insert CEN4-ARS1 fragment [PvuII - CEN4 - dead EcoRI - ARS1 - HindIII/klenowed] into PvuII site of YIp5. Change CAGCTG to CAGGTG so PvuII site is not regenerated and restriction site numbering of [2] is retained. NCBI gi: 406851 Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli. Related vectors: pBR322, YIp5. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37419
- GenBank
- X70276
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified
Sequence
Published Plasmid Map
