Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
Plasmid: YCp7-32phocat
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 8060
- Notes
- The depositor recommends using YRp7-17camp (ATCC 37630) as the reference for CAT expression and using the CAT assay methodology of Neumann, et al. A 1520 bp NdeI fragment containing CEN6 was cloned into the NdeI site of YRp7-32phocat (ATCC 37625). One of a series (ATCC 37624 through 37634) of Escherichia coli/Saccharomyces cerevisiae shuttle vectors for investigating control of expression in yeast. Restriction digests of the clone give the following sizes (kb): EcoRI--6.6, 1.55; PstI--6.7, 1.35; PvuII--6.2, 2.05. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli RR1, Saccharomyces cerevisiae, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37627
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified