Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: YEp13
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 10639
- Notes
- YEp13 is mitotically stable in cir+ strains. [2] A YE type S. cerevisiae/Escherichia coli shuttle vector for cloning specific genes by transformation of yeast. Transforms leu2- yeast to Leu+ at 10E3 to 10E4 colonies per microgram of DNA. An EcoRI fragment containing the 2 micron origin was cloned into the EcoRI site of pBR322. The resulting plasmid was partially digested with PstI, and a PstI fragment containing yeast LEU2 cloned into the 2 micron PstI site. Restriction digests analyzed on agarose gels give the following sizes (kb): PstI--5.5, 4.0, 0.9; HindIII--10.7. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli C600, E.coli K802, E.coli, S.cerevisiae, Saccharomyces. Related vectors: pBR322, 2micron plasmid B form, YEp351, YEp352, YEp356, YEp366, YEp24. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37115
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified