Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: YEp354
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 7942
- Notes
- Created by Moore, July 1995, under contract with NCBI. The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): EcoRI-1; SmaI-2; BamHI-2; SalI-2; PstI-3; HindIII-1. Restriction digests of the clone give the following sizes (kb): EcoRI--8.0; HindIII--8.0; SmaI--8.0; BamHI--8.0; PstI--8.0. (ATCC staff) The sequence and reading frame of the multiple cloning sequence is: 5' GAA TTC CCG GGG ATC CGT CGA CCT GCA GCC AAG CTT GCT CCC 3'. The sequence begins with the first nucleotide of the MCS & ends with the CCC for amino acid 8 of beta-galactosidase. One of 3 promoter-cloning, YE type shuttle vectors (ATCC 37725 to ATCC 37727) with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame. Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli, E.coli MC1061. Related vectors: YEp352, pNM481. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37726
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified