Vector Database

Created by Moore, July 1995, under contract with NCBI. The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): EcoRI-1; SmaI-2; BamHI-2; SalI-2; PstI-3; HindIII-1. Restriction digests of the clone give the following sizes (kb): EcoRI--8.0; HindIII--8.0; SmaI--8.0; BamHI--8.0; PstI--8.0. (ATCC staff) The sequence and reading frame of the multiple cloning sequence is: 5' GAA TTC CCG GGG ATC CGT CGA CCT GCA GCC AAG CTT GCT CCC 3'. The sequence begins with the first nucleotide of the MCS & ends with the CCC for amino acid 8 of beta-galactosidase. One of 3 promoter-cloning, YE type shuttle vectors (ATCC 37725 to ATCC 37727) with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame. Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli, E.coli MC1061. Related vectors: YEp352, pNM481. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)