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pHaMDRwt
(Plasmid #10957)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 10957 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHa
  • Backbone size w/o insert (bp) 11000
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MDR1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4000
  • GenBank ID
    M14758
  • Entrez Gene
    ABCB1 (a.k.a. ABC20, CD243, CLCS, GP170, MDR1, P-GP, PGY1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Full-length cDNA, has the wild-type glycine at position 185 on P-glycoprotein (see GenBank ID M14758.1 for the sequence variations at nucleotides 382, 964, 978, 979, 1660 and 2065 that are present in this plasmid) The "Ha" in pHaMDRwt stand for the Harvey sarcoma virus. Vector can confer the complete multidrug-resistance phenotype to various drug sensitive cell lines.

Figure legend for author's map:
Structure of retroviral vector carrying MDR] cDNA.
sites: B, BamHI; E, EcoRI; H, HindIII; S, Stu I; X, Xho
I. bp, Base pairs; amp, ampicillin resistance gene.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHaMDRwt was a gift from Michael Gottesman (Addgene plasmid # 10957 ; http://n2t.net/addgene:10957 ; RRID:Addgene_10957)
  • For your References section:

    A retrovirus carrying an MDR1 cDNA confers multidrug resistance and polarized expression of P-glycoprotein in MDCK cells. Pastan I, Gottesman MM, Ueda K, Lovelace E, Rutherford AV, Willingham MC. Proc Natl Acad Sci U S A. 1988 Jun . 85(12):4486-90. 10.1073/pnas.85.12.4486 PubMed 2898143