Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11919||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4400
Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)1850
Entrez Genecre (a.k.a. P1_gp003)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
pBS448 carries a GFP-cre fusion gene under the control of the Rous sarcoma virus (RSV) LTR/promoter. The GFP moiety carries the S65T mutation for enhanced fluorescence, but is not codon-optimized for mammalian expression. Higher level expression can be obtained using the EF1-alpha promoter plasmid pBS500 having the same structural fusion gene.
Use of the GFPcre fusion gene demonstrated that the Cre protein carries endogenous determinants that target the protein efficiently to the nucleus of eukaryotic cells. Thus the addition of an extraneous NLS is not required. The GFP-tagged cre gene is a convenient tool for directly observing the tissue-specificity of cre expression in transgenic mice.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBS448 RSV-GFPcre was a gift from Brian Sauer (Addgene plasmid # 11919 ; http://n2t.net/addgene:11919 ; RRID:Addgene_11919)
For your References section:Brief expression of a GFP cre fusion gene in embryonic stem cells allows rapid retrieval of site-specific genomic deletions. Gagneten S, Le Y, Miller J, Sauer B. Nucleic Acids Res. 1997 Aug 15. 25(16):3326-31. 10.1093/nar/25.16.3326 PubMed 9241248
Map uploaded by the depositor.