Addgene - LL - hNANOGi Plasmid Data

Plasmid 12196: LL - hNANOGi

Gene/insert name:
NANOG - RNAi
Alt name:
Nanog
Insert size:
55
Species:
H. sapiens (human)
Entrez Gene:
NANOG ()
Vector backbone:
pLL3.7
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, RNAi, Cre/Lox
Backbone size w/o insert (bp):
7650
Cloning site 5':
HpaI
Site destroyed during cloning:
Yes
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
George Daley
Terms and Licenses:
MTA

Comments:

Target sequence: GGGTTAAGCTGTAACATACTT
(NM_024865: bp 1857-1877)

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: High-efficiency RNA interference in human embryonic stem cells. Zaehres et al (Stem Cells. 2005 Mar . 23(3):299-305. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12196" in your Materials and Methods section.