pMSCV-TAP
(Plasmid #12570)

• Purpose: (Empty Backbone)
• Depositing Lab: David Sabatini
• Publication: Frias et al Curr Biol. 2006 Aug 15. ():.

Backbone

• Vector backbone: pMSCV-TAP
• Backbone manufacturer: Modified from Clontech's pMSCV
• Backbone size (bp): 6800
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • TAP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: MSCV primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: TAP was PCR'd from pBSactTAP, a gift from Dr. Matthias Wilm (Heidelberg, Germany)
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A cDNA encoding the TAP tag was PCR-cloned from the plasmid pBSactTAP with the following primers: left primer 5' -GGACAATG ATCAGGCACCATGGCAGGCCTTGCGCAACACG-3' and right primer 5' -CCGGAATTCCCGGCGGCCGCGGGATCCATTCGCTCGAGGGAC CACCACCACCACCAAGTGCCCCGGAGGATGAG-3' . The PCR product was then cut with BclI/EcoRI and inserted into pMSCV-puro (Clontech) cut with BglII/EcoRI. See author's map for more information (Note that the NotI site is not precisely underlined in the author's map).