Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13464||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameIRES-Bgeo-pA cassette flanked by Fbx15 homologous arms
SpeciesM. musculus (mouse)
Insert Size (bp)10000
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (destroyed during cloning)
- 3′ cloning site SpeI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pTV-Fbx15 was a gift from Shinya Yamanaka (Addgene plasmid # 13464 ; http://n2t.net/addgene:13464 ; RRID:Addgene_13464)
For your References section:Fbx15 is a novel target of Oct3/4 but is dispensable for embryonic stem cell self-renewal and mouse development. Tokuzawa Y, Kaiho E, Maruyama M, Takahashi K, Mitsui K, Maeda M, Niwa H, Yamanaka S. Mol Cell Biol. 2003 Apr . 23(8):2699-708. 10.1128/MCB.23.8.2699-2708.2003 PubMed 12665572
Map uploaded by the depositor.