Addgene - pBV-Luc/Frag-F Plasmid Data

Gene/insert name:
c-myc promoter (-109/-3)
Alt name:
c-myc promoter
Insert size:
106
Species:
H. sapiens (human)
Entrez Gene:
MYC (MRTL, bHLHe39, c-Myc)
Mutation:
-109/-3 (relative to the P2 transcription initiation site) of the c-myc promoter.
Vector backbone:
pBV-luciferase
(Search Vector Database)
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert:
4900
Cloning site 5':
KpnI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Joan Massague
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

-109/-3 (relative to the P2 transcription initiation site) of the c-myc promoter were amplified by PCR and subcloned into the KpnI/EcoRI site of pBV-Luc, a low basal activity luciferase reporter plasmid.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Defective repression of c-myc in breast cancer cells: A loss at the core of the transforming growth factor beta growth arrest program. Chen et al (Proc Natl Acad Sci U S A. 2001 Jan 30. 98(3):992-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14053" in your Materials and Methods section.