Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14053||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4900
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namec-myc promoter (-109/-3)
Alt namec-myc promoter
SpeciesH. sapiens (human)
Insert Size (bp)106
Mutation-109/-3 (relative to the P2 transcription initiation site) of the c-myc promoter.
Entrez GeneMYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
-109/-3 (relative to the P2 transcription initiation site) of the c-myc promoter were amplified by PCR and subcloned into the KpnI/EcoRI site of pBV-Luc, a low basal activity luciferase reporter plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBV-Luc/Frag-F was a gift from Joan Massague (Addgene plasmid # 14053 ; http://n2t.net/addgene:14053 ; RRID:Addgene_14053)
For your References section:Defective repression of c-myc in breast cancer cells: A loss at the core of the transforming growth factor beta growth arrest program. Chen CR, Kang Y, Massague J. Proc Natl Acad Sci U S A. 2001 Jan 30. 98(3):992-9. 10.1073/pnas.98.3.992 PubMed 11158583
Map uploaded by the depositor.