Addgene - pRK5 Flag PRAS40 Plasmid Data

Plasmid 14950: pRK5 Flag PRAS40

Gene/insert name:
PRAS40
Alt name:
AKT1S1
Insert size:
800
Species:
H. sapiens (human)
Entrez Gene:
AKT1S1 (Lobe, PRAS40)
Fusion protein or tag:
Flag
Terminal:
N terminal on backbone
Vector backbone:
Flag-pRK5
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4700
Cloning site 5':
SalI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
SP6 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
The cDNA for PRAS40 was PCR'd from a clone obtained from Origene.
Sequence: View sequences (1)
Principal Investigator:
David Sabatini
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: PRAS40 is an insulin-regulated inhibitor of the mTORC1 protein kinase. Sancak et al (Mol Cell. 2007 Mar 23. 25(6):903-15. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14950" in your Materials and Methods section.