Addgene - pBS mouse c-Myc Plasmid Data

Plasmid 14972: pBS mouse c-Myc

Gene/insert name:
c-Myc
Insert size:
700
Species:
M. musculus (mouse)
Entrez Gene:
Myc (AU016757, Myc2, Niard, Nird, bHLHe39)
Mutation:
Nucleotides 2578-3294
Vector backbone:
pBluescript
(Search Vector Database)
Backbone manufacturer:
Stratagene
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
2900
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
SP6 List of Sequencing Primers
3' sequencing primer:
T7
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Joan Massague
Terms and Licenses:
MTA

Comments:

See author's map for more information.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Mad upregulation and Id2 repression accompany transforming growth factor (TGF)-beta-mediated epithelial cell growth suppression. Siegel et al (J Biol Chem. 2003 Sep 12. 278(37):35444-50. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14972" in your Materials and Methods section.