Addgene - FCK-ChR2-GFP Plasmid Data

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Sequence

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From this article

Edward Boyden Lab Plasmids

This is commonly
requested with

FCK-Halo-GFP

pCAGGS-ChR2- Venus

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Plasmid 15814: FCK-ChR2-GFP

Gene/insert name:		ChR2
Alternative names:		Channelrhodopsin-2
Insert size (bp):		1000
Species of gene(s):		H. sapiens (human)
Fusion proteins or tags:		GFP
Terminal:		C terminal on insert
Vector backbone:		FCK(1.3)GW (Search Vector Database)
Backbone manufacturer:		Pavel Osten
Type of vector:		Mammalian expression,Lentiviral,Bacterial expression
Backbone size (bp):		9500
Cloning site 5':		BamHI
Site destroyed during cloning:		No
Cloning site 3':		AgeI
Site destroyed during cloning:		No
5' Sequencing primer:		na (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		Don't Know
Grow in standard E. coli @ 37C:		No
Please specify bacterial strain for growth and growth condition:		Use recombinase-free E. coli (e.g., Invitrogen's Stbl3)
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		Stbl3
Principal Investigator:		Edward Boyden
Terms and Licenses:		MTA

Comments: See Author's Map for more information. On the Author's Map, "nonstartcodon" refers to the deleted start codon on GFP. This deletion helps reduce background fluorescence.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Selected features

CAG_enhancer

318 - 605

CMV_immearly_promoter

239 - 815

CMV_fwd_primer

772 - 792

HIV-1_5_LTR

835 - 1015

truncHIV-1_3_LTR

835 - 1015

HIV-1_psi_pack

1126 - 1170

RRE

1680 - 1913

Orf frame 1

1558 - 2445

cPPT

2444 - 2459

Orf frame 1

3745 - 5571

EGFP_N_primer

4918 - 4897

EGFP

4864 - 5568

EGFP_C_primer

5505 - 5526

WPRE

5598 - 6185

pBluescriptKS_primer

6204 - 6188

Orf frame 1

5686 - 6735

cPPT

6372 - 6387

U3PPT

6372 - 6393

HIV-1_5_LTR

6709 - 6889

truncHIV-1_3_LTR

6709 - 6889

BGH_rev_primer

6932 - 6915

bGH_PA_terminator

6918 - 7145

f1_origin

7208 - 7514

pBABE_3_primer

7648 - 7628

SV40_enhancer

7849 - 7634

SV40_promoter

7646 - 7914

SV40_origin

7813 - 7890

SV40pro_F_primer

7875 - 7894

EM7_promoter

8008 - 8075

bleo

8076 - 8447

sh_ble

8076 - 8450

SV40_PA_terminator

8583 - 8702

EBV_rev_primer

8671 - 8690

M13_reverse_primer

8764 - 8746

M13_pUC_rev_primer

8785 - 8763

lac_promoter

8828 - 8799

pBR322_origin

9756 - 9137

Orf frame 2

10771 - 9911

Ampicillin

10771 - 9911

AmpR_promoter

10841 - 10813

Unique restriction sites

SpeI		252
NdeI		487
NarI		1019
NotI		1525
XbaI		2651
BamHI		3895
AgeI		4842
EcoRI		5572
SacII		6099
KpnI		6322
MscI		8079
FspI		10206

Please acknowledge the principal investigator if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 15814" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.