pcDNA 3.1(-) VSFP2.1
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16255||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepcDNA3.1 (-)
- Backbone size w/o insert (bp) 5317
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Alt nameVoltage-Sensitive Fluorescent Protein 2.1
Insert Size (bp)2253
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe cDNA coding sequence for Ci-VSP was a gift from Dr. Okamura (NIPS, Okasaki, Japan). The cDNA coding sequence for Cerulean was a gift from Dr. Piston (Vanderbilt University, USA). The cDNA coding sequence for Citrine was a gift from Dr. Griesbeck (MPI Martinsried, Germany).
Terms and Licenses
- Not Available to Industry
Article Citing this Plasmid
Voltage sensor domain of Ci-VSP fused to a pair of fluorescent proteins consisting of a C-terminally truncated CFP (Cerulean) and YFP (Citrine)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA 3.1(-) VSFP2.1 was a gift from Thomas Knopfel (Addgene plasmid # 16255 ; http://n2t.net/addgene:16255 ; RRID:Addgene_16255)
For your References section:Engineering and characterization of an enhanced fluorescent protein voltage sensor. Dimitrov D, He Y, Mutoh H, Baker BJ, Cohen L, Akemann W, Knoepfel T. PLoS ONE. 2007 . 2(5):e440. 10.1371/journal.pone.0000440 PubMed 17487283