pcDNA 3.1(-) VSFP3.1
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18951||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5358
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Alt nameVoltage-Sensitive Fluorescent Protein 3.1
Insert Size (bp)1445
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
As reported in the accompanying publication (Lundby et al., PLoS ONE, 2008 Jun 25;3(6)), the R217Q mutation has been intently introduced in the S4 domain of Ci-VSP to shift the activation curve of the sensor into the physiological range of neuronal membrane potential.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA 3.1(-) VSFP3.1 was a gift from Thomas Knopfel (Addgene plasmid # 18951 ; http://n2t.net/addgene:18951 ; RRID:Addgene_18951)
For your References section:Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements. Lundby A, Mutoh H, Dimitrov D, Akemann W, Knoepfel T. PLoS ONE. 2008 . 3(6):e2514. 10.1371/journal.pone.0002514 PubMed 18575613