Addgene - pB1H2w2-12-C81S Plasmid Data

Plasmid 18042: pB1H2w2-12-C81S

Gene/insert name:
HhaI C81S mutant
Insert size:
1031
Species:
mutant
Mutation:
Simply used as an insert to drop out between Kpn1 and Xba1.
Vector backbone:
ColE1
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
3878
Cloning site 5':
Kpn1
Site destroyed during cloning:
No
Cloning site 3':
Xba1
Site destroyed during cloning:
No
5' sequencing primer:
OMG5 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Principal Investigator:
Scot Wolfe
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A systematic characterization of factors that regulate Drosophila segmentation via a bacterial one-hybrid system. Noyes et al (Nucleic Acids Res. 2008 Mar 10. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18042" in your Materials and Methods section.