pRET.IL.IRES-EGFP (No HSV-TK)
(Plasmid #1836)

• Purpose: (Empty Backbone)
• Depositing Lab: Philip Leder
• Publication: Ishida et al Nucleic Acids Res 1999 Dec 15;27(24):e35.

Backbone

• Vector backbone: pRET
• Vector type: Mammalian Expression, Retroviral, Cre/Lox
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Alt name: retroviral RET construct
• Alt name: removable exon trap
• Alt name: Type I vector

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: na

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See scanned map (#4).
The HSV-TK cassette is eliminated from this vector. Virus production should be fine, but you can not titrate the virus accurately because of the lack of the HSV-TK cassette, the only constitutive marker in RET vectors. You can easily excise all the essential components as an XbaI-XbaI fragment. To obtain larger numbers of G418-resistant clones, this vector carries a strong NEO promoter. This RET vector would be the first choice for making gene-disrupted mice by using transfected or infected ES cells. (Leder #C-1023)