IRES-creERT2-FNF
(Plasmid #188230)

• Purpose: EMCV IRES-cre-ERT2-FRT-Neo*-FRT cassette
• Depositing Lab: Mario Capecchi
• Publication: Additional gene targeting vector construction plasmids (unpublished)

Backbone

• Vector backbone: Custom
• Vector type: Mammalian Expression, Bacterial Expression, Mouse Targeting, Cre/Lox
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: IRES-creERT2-FNF
• Species: Synthetic

Resource Information

• Supplemental Documents:
  • IRES-creERT2+FNF+Id1 arms.gb
  • Alignment to Bochkov and Palmenberg EMCV IRES.pdf
• A portion of this plasmid was derived from a plasmid made by: EMCV IRES is from Peter Mombaerts. cre-ERT2 is from Pierre Chambon. FNF is from Addgene #22687.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This IRES-creERT2-FNF cassette was used in the Id1-IRES-creERT2 allele (http://www.informatics.jax.org/allele/MGI:4366863).

Compared to Bochkov and Palmenberg, 2006 (https://doi.org/10.2144/000112243), this EMCV IRES has a 1-bp deletion and an additional base of A ("7A"). The reason for these changes is unclear. These changes were in the electronic sequence file I received. The actual DNA sequence of the IRES was the same as the electronic sequence file. I incorporated this IRES into my plasmids without introducing additional changes. It was more recently tested in driving tdTomato expression (see https://www.addgene.org/185966/), and it was functional. I did not change those bases back and compare whether the expression level is then higher or lower. It may or may not be attenuated compared to the Bochkov and Palmenberg EMCV IRES.