Addgene - MSCV P2Gm p53 Plasmid Data

Plasmid 19751: MSCV P2Gm p53

Gene/insert name:
oligo targeting p53
Alt name:
p53
Insert size:
100
Species:
M. musculus (mouse)
Entrez Gene:
Trp53 (RP23-56I20.1, Tp53, bbl, bfy, bhy, p44, p53)
Vector backbone:
MSCV P2Gm
(Search Vector Database)
Backbone manufacturer:
MSCV2.2 with WPRE cloned into ClaI site is the base vector
Vector type:
Mammalian Expression, Retroviral, RNAi
Backbone size w/o insert (bp):
7519
Cloning site 5':
XhoI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
GFP miR primer (GCTGGAGTTCGTGACCGCC) List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
30
Growth instructions:
grow in stbl3 cells at 30oC
High or low copy:
Unknown
Selectable markers:
Puromycin
Sequence: View sequences (2)
Map:
View map
Supplemental document:
FLIP vector protocols (application/pdf)
Principal Investigator:
Richard Hynes
Terms and Licenses:
MTA

Comments:

See FLIP vector protocols (PDF from this page) for more information.

There are minor discrepancies between the depositor's sequence and Addgene's sequencing results. The mismatches are either coding joints or a sequencing difference between the actual and the published miR30 vector sequence.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A system for Cre-regulated RNA interference in vivo. Stern et al (Proc Natl Acad Sci U S A. 2008 Sep 16. 105(37):13895-900. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19751" in your Materials and Methods section.