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pSIN4-EF2-O2S
(Plasmid #21162)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21162 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSIN4-EF2-IRES-Pur
  • Backbone size w/o insert (bp) 6300
  • Vector type
    Lentiviral ; reprogramming

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Oct4, Sox2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2037
  • GenBank ID
    NM_002701 NM_003106
  • Entrez Gene
    SOX2 (a.k.a. ANOP3, MCOPS3)
  • Entrez Gene
    POU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer EF1a_for
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

After lentivirus infection, Oct4 and Sox2 are coexpressed by EF2 promoter and IRES intron. Infection with this lentivirus, when used with pSIN4-EF2-N2L and pSIN4-CMV-K2M have a significantly greater reprogramming efficiency than our original 4 lentiviruses.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSIN4-EF2-O2S was a gift from James Thomson (Addgene plasmid # 21162)
  • For your References section:

    Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences. Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA. Science. 2009 Mar 26. 10.1126/science.1172482 PubMed 19325077