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pSIN4-CMV-K2M
(Plasmid #21164)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21164 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSIN4-EF2-IRES-Pur
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression, Lentiviral ; reprogramming

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Klf4, cMyc
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2733
  • GenBank ID
    NM_004235.2 NM_002467
  • Entrez Gene
    MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
  • Entrez Gene
    KLF4 (a.k.a. EZF, GKLF)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer EF1a_for
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

After lentivirus infection, Klf4 and cMyc are coexpressed by CMV promoter and IRES intron. Infections with this lentivirus, when used with pSIN4-EF2-O2S and pSIN4-EF2-N2L have a significantly greater reprogramming efficiency than our original 4 lentiviruses.

NOTE: Addgene's quality control sequence found a K269T in Klf4. This mutation is not known to affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSIN4-CMV-K2M was a gift from James Thomson (Addgene plasmid # 21164 ; http://n2t.net/addgene:21164 ; RRID:Addgene_21164)
  • For your References section:

    Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences. Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA. Science. 2009 Mar 26. 10.1126/science.1172482 PubMed 19325077