pGL2 enhancer- F3 Sp-1 mut
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22432||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepGL2 enhancer
- Backbone size w/o insert (bp) 5854
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Gene/Insert nameF3 Sp-1 mut
SpeciesH. sapiens (human)
Insert Size (bp)1055
/ Fusion Protein
- luciferase reporter gene (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer see article (Common Sequencing Primers)
Forward primer used to create Sp-1 mutation: GGATAGGGACTGGGCGAGG
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGL2 enhancer- F3 Sp-1 mut was a gift from William Sessa (Addgene plasmid # 22432 ; http://n2t.net/addgene:22432 ; RRID:Addgene_22432)
For your References section:Functional analysis of the human endothelial nitric oxide synthase promoter. Sp1 and GATA factors are necessary for basal transcription in endothelial cells. Zhang R, Min W, Sessa WC. J Biol Chem. 1995 Jun 23. 270(25):15320-6. 10.1074/jbc.270.25.15320 PubMed 7541039