Addgene - CMV-miR-31 sponge Plasmid Data

Plasmid 25025: CMV-miR-31 sponge

Gene/insert name:
CMV-miR-31 sponge
Alt name:
transient miR-31 sponge
Insert size:
200
Vector backbone:
pcDNA5-CMV-d2eGFP
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5000
Cloning site 5':
XhoI
Site destroyed during cloning:
Unknown
Cloning site 3':
ApaI
Site destroyed during cloning:
Unknown
5' sequencing primer:
CMV-d2eGFP-F, TATATCATGGCCGACAAGC List of Sequencing Primers
3' sequencing primer:
BGH-rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

miR-31 sponge generated by cloning oligonucleotides containing seven tandem “bulged” (at positions 9-12) miR-31 binding motifs (AGGCAAGACGAGGCATAGCT) into the XhoI and ApaI sites of the CMV sponge backbone.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A pleiotropically acting microRNA, miR-31, inhibits breast cancer metastasis. Valastyan et al (Cell. 2009 Jun 12. 137(6):1032-46. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25025" in your Materials and Methods section.