Addgene - pBABE-puro-mIR-31 sponge Plasmid Data

Plasmid 22694: pBABE-puro-mIR-31 sponge

Gene/insert name:
miR-31
Alt name:
stable miR-31 sponge
Insert size:
1100
Species:
H. sapiens (human)
Entrez Gene:
MIR31 (MIRN31, hsa-mir-31, miR-31)
Mutation:
miR-31 sponge with 7x tandem binding sites to the microRNA.
Vector backbone:
pBABE-puro
(Search Vector Database)
Backbone manufacturer:
Weinberg lab (Addgene#:1764)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5169
Cloning site 5':
BamHI
Site destroyed during cloning:
Unknown
Cloning site 3':
ApaI
Site destroyed during cloning:
Unknown
5' sequencing primer:
pBabe-5 List of Sequencing Primers
3' sequencing primer:
pBabe-3
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

Useful for stable suppression of miR-31 function.

Original sponge backbone modifed from Ebert et al., Nat. Methods (2007); miR-31 construct and stable design are novel

Repeated sequence is: AGGCAAGACGAGGCATAGCT

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A pleiotropically acting microRNA, miR-31, inhibits breast cancer metastasis. Valastyan et al (Cell. 2009 Jun 12. 137(6):1032-46. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22694" in your Materials and Methods section.